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Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins. / Davydova, Anna; Vorobyeva, Mariya; Bashmakova, Eugenia et al.

In: Analytical Biochemistry, Vol. 570, 01.04.2019, p. 43-50.

Research output: Contribution to journal › Article › peer-review

Harvard

Davydova, A, Vorobyeva, M, Bashmakova, E, Vorobjev, P, Krasheninina, O, Tupikin, A, Kabilov, M, Krasitskaya, V, Frank, L & Venyaminova, A 2019, 'Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins', Analytical Biochemistry, vol. 570, pp. 43-50. https://doi.org/10.1016/j.ab.2019.02.004

APA

Davydova, A., Vorobyeva, M., Bashmakova, E., Vorobjev, P., Krasheninina, O., Tupikin, A., Kabilov, M., Krasitskaya, V., Frank, L., & Venyaminova, A. (2019). Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins. Analytical Biochemistry, 570, 43-50. https://doi.org/10.1016/j.ab.2019.02.004

Vancouver

Davydova A, Vorobyeva M, Bashmakova E, Vorobjev P, Krasheninina O, Tupikin A et al. Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins. Analytical Biochemistry. 2019 Apr 1;570:43-50. doi: 10.1016/j.ab.2019.02.004

Author

Davydova, Anna ; Vorobyeva, Mariya ; Bashmakova, Eugenia et al. / Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins. In: Analytical Biochemistry. 2019 ; Vol. 570. pp. 43-50.

BibTeX

@article{e1c9b68dfc614f619a8a789e88f04b01,
title = "Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins",
abstract = "Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2′-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2′-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.",
author = "Anna Davydova and Mariya Vorobyeva and Eugenia Bashmakova and Pavel Vorobjev and Olga Krasheninina and Alexey Tupikin and Marsel Kabilov and Vasilisa Krasitskaya and Ludmila Frank and Alya Venyaminova",
note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = apr,
day = "1",
doi = "10.1016/j.ab.2019.02.004",
language = "English",
volume = "570",
pages = "43--50",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Development and characterization of novel 2′-F-RNA aptamers specific to human total and glycated hemoglobins

AU - Davydova, Anna

AU - Vorobyeva, Mariya

AU - Bashmakova, Eugenia

AU - Vorobjev, Pavel

AU - Krasheninina, Olga

AU - Tupikin, Alexey

AU - Kabilov, Marsel

AU - Krasitskaya, Vasilisa

AU - Frank, Ludmila

AU - Venyaminova, Alya

N1 - Publisher Copyright: © 2019 Elsevier Inc.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2′-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2′-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.

AB - Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2′-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2′-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.

UR - http://www.scopus.com/inward/record.url?scp=85061556058&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2019.02.004

DO - 10.1016/j.ab.2019.02.004

M3 - Article

C2 - 30742800

AN - SCOPUS:85061556058

VL - 570

SP - 43

EP - 50

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

ER -

ID: 18562297