TY - JOUR

T1 - Deuterated stearic acid uptake and accumulation in lipid droplets of cat oocytes

AU - Ranneva, S. V.

AU - Okotrub, K. A.

AU - Amstislavsky, S. Y.

AU - Surovtsev, N. V.

N1 - Publisher Copyright: © 2020 Elsevier Inc.

PY - 2020/10/15

Y1 - 2020/10/15

N2 - Fatty acid uptake and accumulation in lipid droplets are essential processes of lipid metabolism. Oocyte in vitro culture in media enriched with fatty acid is used to modify the lipid content and composition, aiming to study the consequences of obesity and enhance cell cryotolerance. We applied Raman spectroscopy and deuterium labeling approach to quantify stearic acid uptake and investigate its incorporation within oocytes. Our data suggest that deuterium labeling does not affect oocyte maturation rates. The efficiency of deuterated stearic acid (dSA) uptake was shown to decrease with the increase of its concentration in culture medium and the duration of in vitro culture. The molar ratio between dSA and bovine serum albumin has no significant effect on the dSA uptake for 200 μM but modifies concentration dependence of the lipid uptake. dSA accumulates in all the lipid droplets inside oocytes. Different lipid droplets within the same oocyte exhibit different concentrations of dSA. The scatter in the dSA concentration in lipid droplets decreases with the culture time. Using dSA as an example, we provide a comprehensive description of how fatty acid concentration, its molar ratio versus bovine serum albumin, and culture time affect the uptake of the fatty acids in oocytes. Raman microspectroscopy of deuterium-labeled fatty acids is a nondestructive tool providing information about fatty acid uptake and heterogeneity of their accumulation between lipid droplets within the single oocyte.

AB - Fatty acid uptake and accumulation in lipid droplets are essential processes of lipid metabolism. Oocyte in vitro culture in media enriched with fatty acid is used to modify the lipid content and composition, aiming to study the consequences of obesity and enhance cell cryotolerance. We applied Raman spectroscopy and deuterium labeling approach to quantify stearic acid uptake and investigate its incorporation within oocytes. Our data suggest that deuterium labeling does not affect oocyte maturation rates. The efficiency of deuterated stearic acid (dSA) uptake was shown to decrease with the increase of its concentration in culture medium and the duration of in vitro culture. The molar ratio between dSA and bovine serum albumin has no significant effect on the dSA uptake for 200 μM but modifies concentration dependence of the lipid uptake. dSA accumulates in all the lipid droplets inside oocytes. Different lipid droplets within the same oocyte exhibit different concentrations of dSA. The scatter in the dSA concentration in lipid droplets decreases with the culture time. Using dSA as an example, we provide a comprehensive description of how fatty acid concentration, its molar ratio versus bovine serum albumin, and culture time affect the uptake of the fatty acids in oocytes. Raman microspectroscopy of deuterium-labeled fatty acids is a nondestructive tool providing information about fatty acid uptake and heterogeneity of their accumulation between lipid droplets within the single oocyte.

KW - Cat oocyte

KW - Deuteration

KW - Fatty acid

KW - Lipid droplet

KW - Lipid storage

KW - Raman spectroscopy

KW - HUMAN MACROPHAGES

KW - MALDI

KW - CRYOSURVIVAL

KW - RAMAN

KW - FATTY-ACIDS

KW - MAMMALIAN OOCYTES

KW - MATURATION

KW - MASS-SPECTROMETRY

KW - BOVINE BLASTOCYSTS

KW - METABOLISM

UR - http://www.scopus.com/inward/record.url?scp=85089481728&partnerID=8YFLogxK

U2 - 10.1016/j.abb.2020.108532

DO - 10.1016/j.abb.2020.108532

M3 - Article

C2 - 32795451

AN - SCOPUS:85089481728

VL - 692

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

M1 - 108532

ER -