Research output: Contribution to journal › Article › peer-review
Deterministic versus stochastic model of reprogramming : New evidence from cellular barcoding technique. / Yunusova, Anastasia M.; Fishman, Veniamin S.; Vasiliev, Gennady V. et al.
In: Open Biology, Vol. 7, No. 4, 160311, 04.2017.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Deterministic versus stochastic model of reprogramming
T2 - New evidence from cellular barcoding technique
AU - Yunusova, Anastasia M.
AU - Fishman, Veniamin S.
AU - Vasiliev, Gennady V.
AU - Battulin, Nariman R.
N1 - © 2017 The Authors.
PY - 2017/4
Y1 - 2017/4
N2 - Factor-mediated reprogramming of somatic cells towards pluripotency is a low-efficiency process during which only small subsets of cells are successfully reprogrammed. Previous analyses of the determinants of the reprogramming potential are based on average measurements across a large population of cells or on monitoring a relatively small number of single cells with live imaging. Here, we applied lentiviral genetic barcoding, a powerful tool enabling the identification of familiar relationships in thousands of cells. High-throughput sequencing of barcodes from successfully reprogrammed cells revealed a significant number of barcodes from related cells. We developed a computer model, according to which a probability of synchronous reprogramming of sister cells equals 10-30%. We conclude that the reprogramming success is pre-established in some particular cells and, being a heritable trait, can be maintained through cell division. Thus, reprogramming progresses in a deterministic manner, at least at the level of cell lineages.
AB - Factor-mediated reprogramming of somatic cells towards pluripotency is a low-efficiency process during which only small subsets of cells are successfully reprogrammed. Previous analyses of the determinants of the reprogramming potential are based on average measurements across a large population of cells or on monitoring a relatively small number of single cells with live imaging. Here, we applied lentiviral genetic barcoding, a powerful tool enabling the identification of familiar relationships in thousands of cells. High-throughput sequencing of barcodes from successfully reprogrammed cells revealed a significant number of barcodes from related cells. We developed a computer model, according to which a probability of synchronous reprogramming of sister cells equals 10-30%. We conclude that the reprogramming success is pre-established in some particular cells and, being a heritable trait, can be maintained through cell division. Thus, reprogramming progresses in a deterministic manner, at least at the level of cell lineages.
KW - Cell fate decisions
KW - Cellular barcoding
KW - Induced pluripotent stem cells
KW - Reprogramming to pluripotency
KW - Fibroblasts/cytology
KW - Cells, Cultured
KW - Transcription Factors/genetics
KW - Sequence Analysis, DNA
KW - Time-Lapse Imaging
KW - Cellular Reprogramming
KW - Induced Pluripotent Stem Cells/cytology
KW - Animals
KW - Doxycycline/pharmacology
KW - Models, Biological
KW - Recombinant Fusion Proteins/biosynthesis
KW - Genetic Vectors/genetics
KW - Embryo, Mammalian/cytology
KW - Green Fluorescent Proteins/genetics
KW - High-Throughput Nucleotide Sequencing
KW - Mice
KW - Lentivirus/genetics
KW - STATE
KW - cellular barcoding
KW - PLURIPOTENT STEM-CELLS
KW - GROWTH
KW - SOMATIC-CELLS
KW - MOUSE
KW - EMBRYONIC STEM
KW - cell fate decisions
KW - FIBROBLASTS
KW - GENERATION
KW - induced pluripotent stem cells
KW - DIFFERENTIATION
KW - reprogramming to pluripotency
KW - CLONAL ANALYSIS
UR - http://www.scopus.com/inward/record.url?scp=85019246875&partnerID=8YFLogxK
U2 - 10.1098/rsob.160311
DO - 10.1098/rsob.160311
M3 - Article
C2 - 28446707
AN - SCOPUS:85019246875
VL - 7
JO - Open Biology
JF - Open Biology
SN - 2046-2441
IS - 4
M1 - 160311
ER -
ID: 10192770