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Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation. / Gordukova, M. A.; Korsunsky, I. A.; Chursinova, Yu V. et al.

In: Medical Immunology (Russia), Vol. 21, No. 3, 01.01.2019, p. 527-538.

Research output: Contribution to journalArticlepeer-review

Harvard

Gordukova, MA, Korsunsky, IA, Chursinova, YV, Byakhova, MM, Oscorbin, IP, Prodeus, AP & Filipenko, ML 2019, 'Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation', Medical Immunology (Russia), vol. 21, no. 3, pp. 527-538. https://doi.org/10.15789/1563-0625-2019-3-527-538

APA

Gordukova, M. A., Korsunsky, I. A., Chursinova, Y. V., Byakhova, M. M., Oscorbin, I. P., Prodeus, A. P., & Filipenko, M. L. (2019). Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation. Medical Immunology (Russia), 21(3), 527-538. https://doi.org/10.15789/1563-0625-2019-3-527-538

Vancouver

Gordukova MA, Korsunsky IA, Chursinova YV, Byakhova MM, Oscorbin IP, Prodeus AP et al. Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation. Medical Immunology (Russia). 2019 Jan 1;21(3):527-538. doi: 10.15789/1563-0625-2019-3-527-538

Author

Gordukova, M. A. ; Korsunsky, I. A. ; Chursinova, Yu V. et al. / Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation. In: Medical Immunology (Russia). 2019 ; Vol. 21, No. 3. pp. 527-538.

BibTeX

@article{6058b7f926cc4363b7c0c89478afa0f9,
title = "Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation",
abstract = "In this work, we used a reference population of newborns and sampled dried blood spots on Guthrie cards of 2,739 individual samples to determine the reference intervals for TRECs and KRECs values, in order to diagnose primary immunodeficiency by means of neonatal screening. The median absolute values for TRECs and KRECs were 195 (CI95%: 185-206) and 185 (CI95%: 176-197) copies per μl, respectively; the normalized value for TRECs was 2780 (CI95%: 2690-2840), and for KRECs, 2790 (CI95%: 2700-2900) copies per 2 × 105 copies of the albumin gene or 105 cells. The reference interval was calculated for 99 and 99.9 percentiles of total TRECs and KRECs individual values. Due to asymmetric distribution of data, the outliers were filtered off, using the Tukey{\textquoteright}s criterion applied after logarithmic transformation of the data. When analyzing absolute values for TREC/KREC (per μL of blood), no “drop-down” TRECs values were identified; for KRECs, 18 experimental values were excluded from further analysis (from 9.8 to 13.5). The outlying values were not identified among the normalized values of TRECs/KRECs. The obtained reference values for TRECs and KRECs (at the 0.1 percentile level) were, respectively, 458 and 32 per 105 cells, or 23 and 17 per μl of blood samples from neonates.",
keywords = "B cells, KREC, Newborn, Primary immunodeficiency (PID), Reference intervals, Screening, T cells, TREC",
author = "Gordukova, {M. A.} and Korsunsky, {I. A.} and Chursinova, {Yu V.} and Byakhova, {M. M.} and Oscorbin, {I. P.} and Prodeus, {A. P.} and Filipenko, {M. L.}",
note = "Publisher Copyright: {\textcopyright} 2019, SPb RAACI.",
year = "2019",
month = jan,
day = "1",
doi = "10.15789/1563-0625-2019-3-527-538",
language = "English",
volume = "21",
pages = "527--538",
journal = "Medical Immunology (Russia)",
issn = "1563-0625",
publisher = "Russian Association of Allergologists and Clinical Immunologists, St. Petersburg Regional Branch (SPb RAACI)",
number = "3",

}

RIS

TY - JOUR

T1 - Determining reference ranges for TREC and KREC assays in immune deficiency screening of newborns in Russian Federation

AU - Gordukova, M. A.

AU - Korsunsky, I. A.

AU - Chursinova, Yu V.

AU - Byakhova, M. M.

AU - Oscorbin, I. P.

AU - Prodeus, A. P.

AU - Filipenko, M. L.

N1 - Publisher Copyright: © 2019, SPb RAACI.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - In this work, we used a reference population of newborns and sampled dried blood spots on Guthrie cards of 2,739 individual samples to determine the reference intervals for TRECs and KRECs values, in order to diagnose primary immunodeficiency by means of neonatal screening. The median absolute values for TRECs and KRECs were 195 (CI95%: 185-206) and 185 (CI95%: 176-197) copies per μl, respectively; the normalized value for TRECs was 2780 (CI95%: 2690-2840), and for KRECs, 2790 (CI95%: 2700-2900) copies per 2 × 105 copies of the albumin gene or 105 cells. The reference interval was calculated for 99 and 99.9 percentiles of total TRECs and KRECs individual values. Due to asymmetric distribution of data, the outliers were filtered off, using the Tukey’s criterion applied after logarithmic transformation of the data. When analyzing absolute values for TREC/KREC (per μL of blood), no “drop-down” TRECs values were identified; for KRECs, 18 experimental values were excluded from further analysis (from 9.8 to 13.5). The outlying values were not identified among the normalized values of TRECs/KRECs. The obtained reference values for TRECs and KRECs (at the 0.1 percentile level) were, respectively, 458 and 32 per 105 cells, or 23 and 17 per μl of blood samples from neonates.

AB - In this work, we used a reference population of newborns and sampled dried blood spots on Guthrie cards of 2,739 individual samples to determine the reference intervals for TRECs and KRECs values, in order to diagnose primary immunodeficiency by means of neonatal screening. The median absolute values for TRECs and KRECs were 195 (CI95%: 185-206) and 185 (CI95%: 176-197) copies per μl, respectively; the normalized value for TRECs was 2780 (CI95%: 2690-2840), and for KRECs, 2790 (CI95%: 2700-2900) copies per 2 × 105 copies of the albumin gene or 105 cells. The reference interval was calculated for 99 and 99.9 percentiles of total TRECs and KRECs individual values. Due to asymmetric distribution of data, the outliers were filtered off, using the Tukey’s criterion applied after logarithmic transformation of the data. When analyzing absolute values for TREC/KREC (per μL of blood), no “drop-down” TRECs values were identified; for KRECs, 18 experimental values were excluded from further analysis (from 9.8 to 13.5). The outlying values were not identified among the normalized values of TRECs/KRECs. The obtained reference values for TRECs and KRECs (at the 0.1 percentile level) were, respectively, 458 and 32 per 105 cells, or 23 and 17 per μl of blood samples from neonates.

KW - B cells

KW - KREC

KW - Newborn

KW - Primary immunodeficiency (PID)

KW - Reference intervals

KW - Screening

KW - T cells

KW - TREC

UR - http://www.scopus.com/inward/record.url?scp=85070210761&partnerID=8YFLogxK

UR - https://www.elibrary.ru/item.asp?id=38529217

U2 - 10.15789/1563-0625-2019-3-527-538

DO - 10.15789/1563-0625-2019-3-527-538

M3 - Article

AN - SCOPUS:85070210761

VL - 21

SP - 527

EP - 538

JO - Medical Immunology (Russia)

JF - Medical Immunology (Russia)

SN - 1563-0625

IS - 3

ER -

ID: 21254722