Research output: Contribution to journal › Article › peer-review
Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic). / Kartashov, Mikhail Yu; Glushkova, Ludmila I.; Mikryukova, Tamara P. et al.
In: Ticks and Tick-borne Diseases, Vol. 8, No. 4, 01.06.2017, p. 588-592.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Detection of Rickettsia helvetica and Candidatus R. tarasevichiae DNA in Ixodes persulcatus ticks collected in Northeastern European Russia (Komi Republic)
AU - Kartashov, Mikhail Yu
AU - Glushkova, Ludmila I.
AU - Mikryukova, Tamara P.
AU - Korabelnikov, Igor V.
AU - Egorova, Yulia I.
AU - Tupota, Natalia L.
AU - Protopopova, Elena V.
AU - Konovalova, Svetlana N.
AU - Ternovoi, Vladimir A.
AU - Loktev, Valery B.
N1 - Copyright © 2017 Elsevier GmbH. All rights reserved.
PY - 2017/6/1
Y1 - 2017/6/1
N2 - The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in 51 (7.6%) ticks. The nucleotide sequence analysis of gltA fragments (765 bp) from 51 ticks indicated that 60.8% and 39.2% of the ticks were infected with Rickettsia helvetica and Candidatus R. tarasevichiae, respectively. The gltA fragments showed 100% identity with those of Candidatus R. tarasevichiae previously discovered in Siberia and China, whereas R. helvetica showed 99.9% sequence identity with European isolates. The ompB had 8 nucleotide substitutions, 6 of which resulted in amino acid substitutions. In the sca9 gene, 3 nucleotide substitutions were detected, and only one resulted in amino acid substitution. The smpA, ompW, and β-lactamase genes of R. helvetica also showed a high level of sequence identity.
AB - The number of tick-borne infections in the northern European regions of Russia has increased considerably in the last years. In the present study, 676 unfed adult Ixodes persulcatus ticks were collected in the Komi Republic from 2011 to 2013 to study tick-borne rickettsioses. Rickettsia spp. DNA was detected by PCR in 51 (7.6%) ticks. The nucleotide sequence analysis of gltA fragments (765 bp) from 51 ticks indicated that 60.8% and 39.2% of the ticks were infected with Rickettsia helvetica and Candidatus R. tarasevichiae, respectively. The gltA fragments showed 100% identity with those of Candidatus R. tarasevichiae previously discovered in Siberia and China, whereas R. helvetica showed 99.9% sequence identity with European isolates. The ompB had 8 nucleotide substitutions, 6 of which resulted in amino acid substitutions. In the sca9 gene, 3 nucleotide substitutions were detected, and only one resulted in amino acid substitution. The smpA, ompW, and β-lactamase genes of R. helvetica also showed a high level of sequence identity.
KW - Candidatus R. tarasevichiae
KW - Ixodes persulcatus
KW - Komi Republic
KW - Rickettsia helvetica
KW - KAZAKSTAN
KW - WESTERN SIBERIA
KW - HEILONGJIANGENSIS
KW - IDENTIFICATION
KW - SPOTTED-FEVER GROUP
KW - EHRLICHIA
KW - TOMSK CITY
KW - NORTH
KW - ENCEPHALITIS
KW - BORNE RICKETTSIOSES
KW - Phylogeny
KW - Sequence Analysis, DNA
KW - Rickettsia/classification
KW - Russia
KW - Animals
KW - Bacterial Proteins/genetics
KW - Ixodes/microbiology
UR - http://www.scopus.com/inward/record.url?scp=85017509708&partnerID=8YFLogxK
U2 - 10.1016/j.ttbdis.2017.04.001
DO - 10.1016/j.ttbdis.2017.04.001
M3 - Article
C2 - 28433730
AN - SCOPUS:85017509708
VL - 8
SP - 588
EP - 592
JO - Ticks and Tick-borne Diseases
JF - Ticks and Tick-borne Diseases
SN - 1877-959X
IS - 4
ER -
ID: 10263893