TY - JOUR

T1 - Detection of Babesia caballi, Theileria mutans and Th. velifera in ixodid ticks collected from cattle in Guinea in 2017–2018

AU - Kartashov, Mikhail Yu

AU - Naidenova, Ekaterina V.

AU - Zakharov, Kirill S.

AU - Yakovlev, Sergey A.

AU - Skarnovich, Maxim O.

AU - Boumbaly, Sanaba

AU - Nikiforov, Konstantin A.

AU - Plekhanov, Nikita A.

AU - Kritzkiy, Andrey A.

AU - Ternovoi, Vladimir A.

AU - Boiro, Mamadou Y.

AU - Loktev, Valery B.

N1 - Funding Information: The authors would like to gratefully acknowledge support from the Russian Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing , Project RP № 2904 from 12/22/2017. Publisher Copyright: © 2021 Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/4

Y1 - 2021/4

N2 - Intraerythrocytic protozoan parasites from the genera Babesia and Theileria may infect a wide range of animals and humans. The purpose of this study was to detect the 18S ribosomal RNA gene of Babesia spp. and Theileria spp. in ticks collected from household cows in the Republic of Guinea from 2017 to 2018 by PCR and then genotype the gene fragments by sequencing. A total of 907 ticks from 319 cows were collected in seven prefectures of Guinea (Boke, Faranah, Kankan, Kindia, Labe, Mamou and N'Zerekore). The following tick species on cattle were identified: Amblyomma variegatum (44.2%), Rhipicephalus decoloratus (34.7%), Rh. annulatus (10.3%), Rh. geigyi (7.3%) Hyalomma truncatum (2.4%), Rh. senegalensis (0.8%) and Haemaphysalis leachi (0.6%). Genetic markers for piroplasms were found in Am. variegatum, Rh. decoloratus, Rh. annulatus, and Rh. geigyi ticks, and the total infection rate for these ticks was 4.2%. The highest infection rate was found in Rh. annulatus ticks (10.9%). The piroplasms were genotyped as Babesia caballi, Theileria mutans and Theileria velifera by phylogenetic analysis of the 1150 bp 18S ribosomal RNA gene fragments. These pathogens were discovered in practically all studied prefectures in Guinea except for Mamou Prefecture. We propose that these ixodid ticks might play a major role in the transmission of piroplasm infections in domestic animals in Guinea.

AB - Intraerythrocytic protozoan parasites from the genera Babesia and Theileria may infect a wide range of animals and humans. The purpose of this study was to detect the 18S ribosomal RNA gene of Babesia spp. and Theileria spp. in ticks collected from household cows in the Republic of Guinea from 2017 to 2018 by PCR and then genotype the gene fragments by sequencing. A total of 907 ticks from 319 cows were collected in seven prefectures of Guinea (Boke, Faranah, Kankan, Kindia, Labe, Mamou and N'Zerekore). The following tick species on cattle were identified: Amblyomma variegatum (44.2%), Rhipicephalus decoloratus (34.7%), Rh. annulatus (10.3%), Rh. geigyi (7.3%) Hyalomma truncatum (2.4%), Rh. senegalensis (0.8%) and Haemaphysalis leachi (0.6%). Genetic markers for piroplasms were found in Am. variegatum, Rh. decoloratus, Rh. annulatus, and Rh. geigyi ticks, and the total infection rate for these ticks was 4.2%. The highest infection rate was found in Rh. annulatus ticks (10.9%). The piroplasms were genotyped as Babesia caballi, Theileria mutans and Theileria velifera by phylogenetic analysis of the 1150 bp 18S ribosomal RNA gene fragments. These pathogens were discovered in practically all studied prefectures in Guinea except for Mamou Prefecture. We propose that these ixodid ticks might play a major role in the transmission of piroplasm infections in domestic animals in Guinea.

KW - Babesia caballi

KW - Cattle

KW - Guinea

KW - Theileria mutans

KW - Theileria velifera

KW - Ticks

UR - http://www.scopus.com/inward/record.url?scp=85104126751&partnerID=8YFLogxK

U2 - 10.1016/j.vprsr.2021.100564

DO - 10.1016/j.vprsr.2021.100564

M3 - Article

C2 - 34024380

AN - SCOPUS:85104126751

VL - 24

JO - Veterinary Parasitology: Regional Studies and Reports

JF - Veterinary Parasitology: Regional Studies and Reports

SN - 2405-9390

M1 - 100564

ER -