TY - JOUR

T1 - Delivery of DNA and mRNA vaccines by human milk exosomes

AU - Мартюшова, Владислава Геннадьевна

AU - Timofeeva, A.

AU - Седых, Сергей Евгеньевич

N1 - Delivery of DNA and mRNA vaccines by human milk exosomes / V. Martyushova, A. Timofeeva, S. Sedykh // Posters // FEBS Open Bio. – 2024. – Vol. 14, No. S2. – С. 169. – DOI 10.1002/2211-5463.13837.

PY - 2024/6/26

Y1 - 2024/6/26

N2 - Delivering DNA and mRNA vaccines into the body is still challenging, and there is currently no universal solution. Artificially synthesized liposomes have a number of well-known disadvantages. This work aimed to create a delivery system for mRNA and DNA vaccines (pVAX1) containing the RBD gene in laboratory mice bodies using milk exosomes and auxiliary reagents. To isolate exosomes from milk preparations, we used a previously developed method that included the stages of centrifugation, ultrafiltration, and ultracentrifugation. We also purified exosomes from co-isolating impurities using gel filtration. The resulting preparations were loaded with DNA and mRNA vaccines; laboratory mice of the CD1 line and other strains were immunized with the loaded exosome preparations. The vaccine was administered twice, with an interval of two weeks; two weeks after the second immunization, the animals were slaughtered, and the titer of antibodies against RBD was analyzed. It has been shown that DNA and mRNA vaccine preparations are effectively loaded into milk exosomes. A statistically significant increase in antibody titers to RBD was observed in laboratory animals' blood plasma, comparable to that after the immunization with the RBD with Freund's adjuvant. The results obtained indicate that milk exosomes can be used as delivery vehicles for DNA and mRNA vaccines.

AB - Delivering DNA and mRNA vaccines into the body is still challenging, and there is currently no universal solution. Artificially synthesized liposomes have a number of well-known disadvantages. This work aimed to create a delivery system for mRNA and DNA vaccines (pVAX1) containing the RBD gene in laboratory mice bodies using milk exosomes and auxiliary reagents. To isolate exosomes from milk preparations, we used a previously developed method that included the stages of centrifugation, ultrafiltration, and ultracentrifugation. We also purified exosomes from co-isolating impurities using gel filtration. The resulting preparations were loaded with DNA and mRNA vaccines; laboratory mice of the CD1 line and other strains were immunized with the loaded exosome preparations. The vaccine was administered twice, with an interval of two weeks; two weeks after the second immunization, the animals were slaughtered, and the titer of antibodies against RBD was analyzed. It has been shown that DNA and mRNA vaccine preparations are effectively loaded into milk exosomes. A statistically significant increase in antibody titers to RBD was observed in laboratory animals' blood plasma, comparable to that after the immunization with the RBD with Freund's adjuvant. The results obtained indicate that milk exosomes can be used as delivery vehicles for DNA and mRNA vaccines.

UR - https://febs.onlinelibrary.wiley.com/doi/10.1002/2211-5463.13837

UR - https://www.elibrary.ru/item.asp?id=68344715

U2 - 10.1002/2211-5463.13837

DO - 10.1002/2211-5463.13837

M3 - Conference article

VL - 14

SP - 169

JO - FEBS Open Bio

JF - FEBS Open Bio

SN - 2211-5463

IS - S2

M1 - P-14-018

ER -