Research output: Contribution to journal › Article › peer-review
Decellularization and an In Situ Tissue Engineering Approach in the Development of an Aortic Graft: Technological Features and Mechanobiological Studies. / Sergeevichev, David; Fomenko, Vladislav; Chepeleva, Elena et al.
In: Polymers, Vol. 17, No. 3, 305, 23.01.2025.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Decellularization and an In Situ Tissue Engineering Approach in the Development of an Aortic Graft: Technological Features and Mechanobiological Studies
AU - Sergeevichev, David
AU - Fomenko, Vladislav
AU - Chepeleva, Elena
AU - Kuznetsova, Elena
AU - Vaver, Andrey
AU - Zhulkov, Maxim
AU - Vasiliyeva, Maria
PY - 2025/1/23
Y1 - 2025/1/23
N2 - This study presents a novel method to enhance the biocompatibility of decellularized porcine aortic segments while preserving their mechanical properties and histological structure. Detergent-decellularized aortic segments were treated with modified globular chitosan (Novochizol™) at varying concentrations (0.5%, 1%, 2%, and 3%) by sonication and subsequently subjected to mechanical testing. To further improve cell infiltration, blind-ended laser channels were created within the decellularized segments. The modified grafts were then seeded with porcine vascular interstitial cells in vitro for 7 days or implanted into the thoracic aorta of minipigs for 30 days. Histological analysis was performed at each stage of the study. Impregnation with Novochizol™ significantly increased the specific strength (from 0.97 ± 0.19 MPa to 4.99 ± 2.43 MPa) and Young’s modulus (from 0.73 ± 0.06 MPa to 14.66 ± 7.14 MPa) of the decellularized aortic segments. Histological examination confirmed the preservation of the connective tissue matrix’s morphological structure. Optimal modification conditions were identified as a 30 min sonication in a 1% Novochizol™ solution at 25 °C. A 35 ms continuous laser treatment was sufficient to create a 1 mm deep blind-ended channel, thereby promoting the seeding of vascular interstitial cells within the acellular graft, as confirmed by implantation in minipigs.
AB - This study presents a novel method to enhance the biocompatibility of decellularized porcine aortic segments while preserving their mechanical properties and histological structure. Detergent-decellularized aortic segments were treated with modified globular chitosan (Novochizol™) at varying concentrations (0.5%, 1%, 2%, and 3%) by sonication and subsequently subjected to mechanical testing. To further improve cell infiltration, blind-ended laser channels were created within the decellularized segments. The modified grafts were then seeded with porcine vascular interstitial cells in vitro for 7 days or implanted into the thoracic aorta of minipigs for 30 days. Histological analysis was performed at each stage of the study. Impregnation with Novochizol™ significantly increased the specific strength (from 0.97 ± 0.19 MPa to 4.99 ± 2.43 MPa) and Young’s modulus (from 0.73 ± 0.06 MPa to 14.66 ± 7.14 MPa) of the decellularized aortic segments. Histological examination confirmed the preservation of the connective tissue matrix’s morphological structure. Optimal modification conditions were identified as a 30 min sonication in a 1% Novochizol™ solution at 25 °C. A 35 ms continuous laser treatment was sufficient to create a 1 mm deep blind-ended channel, thereby promoting the seeding of vascular interstitial cells within the acellular graft, as confirmed by implantation in minipigs.
KW - cardiac valve pathology
KW - decellularization
KW - mechanical strength
KW - tissue engineering
KW - xenograft
UR - https://www.mendeley.com/catalogue/d532f56d-f0d9-3785-aaf1-d0ae6f84bbff/
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85217532667&origin=inward&txGid=9f375e64985666a980abea82e210c279
U2 - 10.3390/polym17030305
DO - 10.3390/polym17030305
M3 - Article
C2 - 39940507
VL - 17
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 3
M1 - 305
ER -
ID: 64737289