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Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice. / Ershov, Nikita I.; Bondar, Natalya P.; Lepeshko, Arina A. et al.

In: BMC Genomics, Vol. 19, No. Suppl 3, 93, 09.02.2018, p. 93.

Research output: Contribution to journalArticlepeer-review

Harvard

Ershov, NI, Bondar, NP, Lepeshko, AA, Reshetnikov, VV, Ryabushkina, JA & Merkulova, TI 2018, 'Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice', BMC Genomics, vol. 19, no. Suppl 3, 93, pp. 93. https://doi.org/10.1186/s12864-018-4479-2

APA

Ershov, N. I., Bondar, N. P., Lepeshko, A. A., Reshetnikov, V. V., Ryabushkina, J. A., & Merkulova, T. I. (2018). Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice. BMC Genomics, 19(Suppl 3), 93. [93]. https://doi.org/10.1186/s12864-018-4479-2

Vancouver

Ershov NI, Bondar NP, Lepeshko AA, Reshetnikov VV, Ryabushkina JA, Merkulova TI. Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice. BMC Genomics. 2018 Feb 9;19(Suppl 3):93. 93. doi: 10.1186/s12864-018-4479-2

Author

Ershov, Nikita I. ; Bondar, Natalya P. ; Lepeshko, Arina A. et al. / Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice. In: BMC Genomics. 2018 ; Vol. 19, No. Suppl 3. pp. 93.

BibTeX

@article{6ed7dbcc5df048fdbc1724b77f3e92e7,
title = "Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice",
abstract = "Background: Maternal separation models in rodents are widely used to establish molecular mechanisms underlying prolonged effects of early life adversity on neurobiological and behavioral outcomes in adulthood. However, global epigenetic signatures following early life stress in these models remain unclear. Results: In this study, we carried out a ChIP-seq analysis of H3K4 trimethylation profile in the prefrontal cortex of adult male mice with a history of early life stress. Two types of stress were used: prolonged separation of pups from their mothers (for 3 h once a day, maternal separation, MS) and brief separation (for 15 min once a day, handling, HD). Adult offspring in the MS group demonstrated reduced locomotor activity in the open field test accompanied by reduced exploratory activity, while the HD group showed decreased anxiety-like behavior only. In a group of maternal separation, we have found a small number (45) of slightly up-regulated peaks, corresponding to promoters of 70 genes, while no changes were observed in a group of handling. Among the genes whose promoters have differential enrichment of H3K4me3, the most relevant ones participate in gene expression regulation, modulation of chromatin structure and mRNA processing. For two genes, Ddias and Pip4k2a, increased H3K4me3 levels were associated with the increased mRNA expression in MS group. Conclusion: The distribution of H3K4me3 in prefrontal cortex showed relatively low variability across all individuals, and only some subtle changes were revealed in mice with a history of early life stress. It is possible that the observed long-lasting behavioral alterations induced by maternal separation are mediated by other epigenetic mechanisms, or other brain structures are responsible for these effects.",
keywords = "ChIP-seq, Early life stress, H3K4me3, Handling, Maternal separation, Mice, Prefrontal cortex, Lysine/metabolism, Genomics, Mice, Inbred C57BL, Prefrontal Cortex/metabolism, Male, Emotions, Behavior, Animal, Maternal Deprivation, Stress, Psychological/genetics, Animals, Histones/chemistry, Female, Methylation, Adaptation, Psychological, RATS, HISTONE CHAPERONE SPT6, BRAIN, ANXIETY, BEHAVIOR, C57BL/6 MICE, MAMMALIAN GENOMES, MATERNAL SEPARATION, GENE, WORKING-MEMORY",
author = "Ershov, {Nikita I.} and Bondar, {Natalya P.} and Lepeshko, {Arina A.} and Reshetnikov, {Vasiliy V.} and Ryabushkina, {Julia A.} and Merkulova, {Tatiana I.}",
note = "Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = feb,
day = "9",
doi = "10.1186/s12864-018-4479-2",
language = "English",
volume = "19",
pages = "93",
journal = "BMC Genomics",
issn = "1471-2164",
publisher = "BioMed Central Ltd.",
number = "Suppl 3",

}

RIS

TY - JOUR

T1 - Consequences of early life stress on genomic landscape of H3K4me3 in prefrontal cortex of adult mice

AU - Ershov, Nikita I.

AU - Bondar, Natalya P.

AU - Lepeshko, Arina A.

AU - Reshetnikov, Vasiliy V.

AU - Ryabushkina, Julia A.

AU - Merkulova, Tatiana I.

N1 - Publisher Copyright: © 2018 The Author(s).

PY - 2018/2/9

Y1 - 2018/2/9

N2 - Background: Maternal separation models in rodents are widely used to establish molecular mechanisms underlying prolonged effects of early life adversity on neurobiological and behavioral outcomes in adulthood. However, global epigenetic signatures following early life stress in these models remain unclear. Results: In this study, we carried out a ChIP-seq analysis of H3K4 trimethylation profile in the prefrontal cortex of adult male mice with a history of early life stress. Two types of stress were used: prolonged separation of pups from their mothers (for 3 h once a day, maternal separation, MS) and brief separation (for 15 min once a day, handling, HD). Adult offspring in the MS group demonstrated reduced locomotor activity in the open field test accompanied by reduced exploratory activity, while the HD group showed decreased anxiety-like behavior only. In a group of maternal separation, we have found a small number (45) of slightly up-regulated peaks, corresponding to promoters of 70 genes, while no changes were observed in a group of handling. Among the genes whose promoters have differential enrichment of H3K4me3, the most relevant ones participate in gene expression regulation, modulation of chromatin structure and mRNA processing. For two genes, Ddias and Pip4k2a, increased H3K4me3 levels were associated with the increased mRNA expression in MS group. Conclusion: The distribution of H3K4me3 in prefrontal cortex showed relatively low variability across all individuals, and only some subtle changes were revealed in mice with a history of early life stress. It is possible that the observed long-lasting behavioral alterations induced by maternal separation are mediated by other epigenetic mechanisms, or other brain structures are responsible for these effects.

AB - Background: Maternal separation models in rodents are widely used to establish molecular mechanisms underlying prolonged effects of early life adversity on neurobiological and behavioral outcomes in adulthood. However, global epigenetic signatures following early life stress in these models remain unclear. Results: In this study, we carried out a ChIP-seq analysis of H3K4 trimethylation profile in the prefrontal cortex of adult male mice with a history of early life stress. Two types of stress were used: prolonged separation of pups from their mothers (for 3 h once a day, maternal separation, MS) and brief separation (for 15 min once a day, handling, HD). Adult offspring in the MS group demonstrated reduced locomotor activity in the open field test accompanied by reduced exploratory activity, while the HD group showed decreased anxiety-like behavior only. In a group of maternal separation, we have found a small number (45) of slightly up-regulated peaks, corresponding to promoters of 70 genes, while no changes were observed in a group of handling. Among the genes whose promoters have differential enrichment of H3K4me3, the most relevant ones participate in gene expression regulation, modulation of chromatin structure and mRNA processing. For two genes, Ddias and Pip4k2a, increased H3K4me3 levels were associated with the increased mRNA expression in MS group. Conclusion: The distribution of H3K4me3 in prefrontal cortex showed relatively low variability across all individuals, and only some subtle changes were revealed in mice with a history of early life stress. It is possible that the observed long-lasting behavioral alterations induced by maternal separation are mediated by other epigenetic mechanisms, or other brain structures are responsible for these effects.

KW - ChIP-seq

KW - Early life stress

KW - H3K4me3

KW - Handling

KW - Maternal separation

KW - Mice

KW - Prefrontal cortex

KW - Lysine/metabolism

KW - Genomics

KW - Mice, Inbred C57BL

KW - Prefrontal Cortex/metabolism

KW - Male

KW - Emotions

KW - Behavior, Animal

KW - Maternal Deprivation

KW - Stress, Psychological/genetics

KW - Animals

KW - Histones/chemistry

KW - Female

KW - Methylation

KW - Adaptation, Psychological

KW - RATS

KW - HISTONE CHAPERONE SPT6

KW - BRAIN

KW - ANXIETY

KW - BEHAVIOR

KW - C57BL/6 MICE

KW - MAMMALIAN GENOMES

KW - MATERNAL SEPARATION

KW - GENE

KW - WORKING-MEMORY

UR - http://www.scopus.com/inward/record.url?scp=85041857415&partnerID=8YFLogxK

U2 - 10.1186/s12864-018-4479-2

DO - 10.1186/s12864-018-4479-2

M3 - Article

C2 - 29504911

AN - SCOPUS:85041857415

VL - 19

SP - 93

JO - BMC Genomics

JF - BMC Genomics

SN - 1471-2164

IS - Suppl 3

M1 - 93

ER -

ID: 10422519