Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents

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Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents. / Danilin, N. A.; Matveev, A. L.; Tikunova, N. V. et al.

In: Russian Journal of Bioorganic Chemistry, Vol. 47, No. 2, 03.2021, p. 469-477.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{9a9595b621b44465a4c7d48ff3a73f45,

title = "Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents",

abstract = "The conjugates of RNase P guiding oligo(2'-O-methylribo)- and oligodeoxyribonucleotides (EGS oligonucleotides) with oligo(N-methylpyrrole) have been synthesized for the first time. The ability of RNase P to hydrolyze RNA in the presence of EGS oligonucleotides and their conjugates with oligo(N-methylpyrrole) has been demonstrated in model systems using fluorescently labeled chemically synthesized oligoribonucleotides corresponding to the fragments of mRNA of the ftsZ and gyrA genes of Acinetobacter baumannii. It has been shown that hydrolysis of RNA by RNase P occurs more efficiently in the presence of the conjugates than in the case of unmodified oligodeoxyribonucleotides. The introduction of oligo(N-methylpyrrole) in EGS oligo(2'-O-methylribonucleotides) insignificantly changes the effectiveness of hydrolysis. It has been demonstrated that the cell-penetrating ability of oligonucleotides is enhanced because of the presence of oligo(N-methylpyrrole) at the 5'-end.",

keywords = "bacterial RNAse P, EGS-oligonucleotides, oligo(2'-O-methylribonucleotides), oligo(N-methylpyrrole)",

author = "Danilin, {N. A.} and Matveev, {A. L.} and Tikunova, {N. V.} and Venyaminova, {A. G.} and Novopashina, {D. S.}",

note = "Funding Information: The work was supported by the State-funded budget projects (Program of fundamental scientific research of the State Academies of Sciences for 2013-2020 [АААА-А17-117020210021-7]). Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = mar,

doi = "10.1134/S1068162021020084",

language = "English",

volume = "47",

pages = "469--477",

journal = "Russian Journal of Bioorganic Chemistry",

issn = "1068-1620",

publisher = "MAIK NAUKA/INTERPERIODICA/SPRINGER",

number = "2",

}

RIS

TY - JOUR

T1 - Conjugates of RNase P-Guiding Oligonucleotides with Oligo(N-Methylpyrrole) as Prospective Antibacterial Agents

AU - Danilin, N. A.

AU - Matveev, A. L.

AU - Tikunova, N. V.

AU - Venyaminova, A. G.

AU - Novopashina, D. S.

N1 - Funding Information: The work was supported by the State-funded budget projects (Program of fundamental scientific research of the State Academies of Sciences for 2013-2020 [АААА-А17-117020210021-7]). Publisher Copyright: © 2021, Pleiades Publishing, Ltd. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/3

Y1 - 2021/3

N2 - The conjugates of RNase P guiding oligo(2'-O-methylribo)- and oligodeoxyribonucleotides (EGS oligonucleotides) with oligo(N-methylpyrrole) have been synthesized for the first time. The ability of RNase P to hydrolyze RNA in the presence of EGS oligonucleotides and their conjugates with oligo(N-methylpyrrole) has been demonstrated in model systems using fluorescently labeled chemically synthesized oligoribonucleotides corresponding to the fragments of mRNA of the ftsZ and gyrA genes of Acinetobacter baumannii. It has been shown that hydrolysis of RNA by RNase P occurs more efficiently in the presence of the conjugates than in the case of unmodified oligodeoxyribonucleotides. The introduction of oligo(N-methylpyrrole) in EGS oligo(2'-O-methylribonucleotides) insignificantly changes the effectiveness of hydrolysis. It has been demonstrated that the cell-penetrating ability of oligonucleotides is enhanced because of the presence of oligo(N-methylpyrrole) at the 5'-end.

AB - The conjugates of RNase P guiding oligo(2'-O-methylribo)- and oligodeoxyribonucleotides (EGS oligonucleotides) with oligo(N-methylpyrrole) have been synthesized for the first time. The ability of RNase P to hydrolyze RNA in the presence of EGS oligonucleotides and their conjugates with oligo(N-methylpyrrole) has been demonstrated in model systems using fluorescently labeled chemically synthesized oligoribonucleotides corresponding to the fragments of mRNA of the ftsZ and gyrA genes of Acinetobacter baumannii. It has been shown that hydrolysis of RNA by RNase P occurs more efficiently in the presence of the conjugates than in the case of unmodified oligodeoxyribonucleotides. The introduction of oligo(N-methylpyrrole) in EGS oligo(2'-O-methylribonucleotides) insignificantly changes the effectiveness of hydrolysis. It has been demonstrated that the cell-penetrating ability of oligonucleotides is enhanced because of the presence of oligo(N-methylpyrrole) at the 5'-end.

KW - bacterial RNAse P

KW - EGS-oligonucleotides

KW - oligo(2'-O-methylribonucleotides)

KW - oligo(N-methylpyrrole)

UR - http://www.scopus.com/inward/record.url?scp=85104884955&partnerID=8YFLogxK

U2 - 10.1134/S1068162021020084

DO - 10.1134/S1068162021020084

M3 - Article

AN - SCOPUS:85104884955

VL - 47

SP - 469

EP - 477

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 2

ER -

ID: 28452453