Comparison of isotonic activation of cell volume regulation in rat peritoneal mesothelial cells and in kidney outer medullary collecting duct principal cells

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Comparison of isotonic activation of cell volume regulation in rat peritoneal mesothelial cells and in kidney outer medullary collecting duct principal cells. / Baturina, Galina S.; Katkova, Liubov E.; Schmitt, Claus Peter et al.

In: Biomolecules, Vol. 11, No. 10, 1452, 10.2021.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{2415dbdc3c644dd5bf90c28b614d34c2,

title = "Comparison of isotonic activation of cell volume regulation in rat peritoneal mesothelial cells and in kidney outer medullary collecting duct principal cells",

abstract = "In disease states, mesothelial cells are exposed to variable osmotic conditions, with high osmotic stress exerted by peritoneal dialysis (PD) fluids. They contain unphysiologically high concentrations of glucose and result in major peritoneal membrane transformation and PD function loss. The effects of isotonic entry of urea and myo-inositol in hypertonic (380 mOsm/kg) medium on the cell volume of primary cultures of rat peritoneal mesothelial cells and rat kidney outer medullary collecting duct (OMCD) principal cells were studied. In hypertonic medium, rat peritoneal mesothelial cells activated a different mechanism of cell volume regulation in the presence of isotonic urea (100 mM) in comparison to rat kidney OMCD principal cells. In kidney OMCD cells inflow of urea into the shrunken cell results in restoration of cell volume. In the shrunken peritoneal mesothelial cells, isotonic urea inflow caused a small volume increase and activated regulatory volume decrease (RVD). Isotonic myo-inositol activated RVD in hypertonic medium in both cell types. Isotonic application of both osmolytes caused a sharp increase of intracellular calcium both in peritoneal mesothelial cells and in kidney OMCD principal cells. In conclusion, peritoneal mesothelial cells exhibit RVD mechanisms when challenged with myo-inositol and urea under hyperosmolar isotonic switch from mannitol through involvement of calcium-dependent control. Myo-inositol effects were identical with the ones in OMCD principal cells whereas urea effects in OMCD principal cells led to no RVD induction.",

keywords = "Cell volume regulation, Kidney principal cells, Mesothelial cells, Organic osmolytes, Osmotic stress",

author = "Baturina, {Galina S.} and Katkova, {Liubov E.} and Schmitt, {Claus Peter} and Solenov, {Evgeniy I.} and Zarogiannis, {Sotirios G.}",

note = "Funding Information: Funding: This research was funded by RFBR, grant numbers ## 19-08-00874-a and 20-015-00147-a to G.S.B. and by the International Society of Peritoneal Dialysis through an International Collaboration grant to S.G.Z. for 2019–2021. S.G.Z. acknowledges the Alexander von Humboldt Stiftung for an Experienced Researcher Fellowship for 2019–2021. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = oct,

doi = "10.3390/biom11101452",

language = "English",

volume = "11",

journal = "Biomolecules",

issn = "2218-273X",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "10",

}

RIS

TY - JOUR

T1 - Comparison of isotonic activation of cell volume regulation in rat peritoneal mesothelial cells and in kidney outer medullary collecting duct principal cells

AU - Baturina, Galina S.

AU - Katkova, Liubov E.

AU - Schmitt, Claus Peter

AU - Solenov, Evgeniy I.

AU - Zarogiannis, Sotirios G.

N1 - Funding Information: Funding: This research was funded by RFBR, grant numbers ## 19-08-00874-a and 20-015-00147-a to G.S.B. and by the International Society of Peritoneal Dialysis through an International Collaboration grant to S.G.Z. for 2019–2021. S.G.Z. acknowledges the Alexander von Humboldt Stiftung for an Experienced Researcher Fellowship for 2019–2021. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/10

Y1 - 2021/10

N2 - In disease states, mesothelial cells are exposed to variable osmotic conditions, with high osmotic stress exerted by peritoneal dialysis (PD) fluids. They contain unphysiologically high concentrations of glucose and result in major peritoneal membrane transformation and PD function loss. The effects of isotonic entry of urea and myo-inositol in hypertonic (380 mOsm/kg) medium on the cell volume of primary cultures of rat peritoneal mesothelial cells and rat kidney outer medullary collecting duct (OMCD) principal cells were studied. In hypertonic medium, rat peritoneal mesothelial cells activated a different mechanism of cell volume regulation in the presence of isotonic urea (100 mM) in comparison to rat kidney OMCD principal cells. In kidney OMCD cells inflow of urea into the shrunken cell results in restoration of cell volume. In the shrunken peritoneal mesothelial cells, isotonic urea inflow caused a small volume increase and activated regulatory volume decrease (RVD). Isotonic myo-inositol activated RVD in hypertonic medium in both cell types. Isotonic application of both osmolytes caused a sharp increase of intracellular calcium both in peritoneal mesothelial cells and in kidney OMCD principal cells. In conclusion, peritoneal mesothelial cells exhibit RVD mechanisms when challenged with myo-inositol and urea under hyperosmolar isotonic switch from mannitol through involvement of calcium-dependent control. Myo-inositol effects were identical with the ones in OMCD principal cells whereas urea effects in OMCD principal cells led to no RVD induction.

AB - In disease states, mesothelial cells are exposed to variable osmotic conditions, with high osmotic stress exerted by peritoneal dialysis (PD) fluids. They contain unphysiologically high concentrations of glucose and result in major peritoneal membrane transformation and PD function loss. The effects of isotonic entry of urea and myo-inositol in hypertonic (380 mOsm/kg) medium on the cell volume of primary cultures of rat peritoneal mesothelial cells and rat kidney outer medullary collecting duct (OMCD) principal cells were studied. In hypertonic medium, rat peritoneal mesothelial cells activated a different mechanism of cell volume regulation in the presence of isotonic urea (100 mM) in comparison to rat kidney OMCD principal cells. In kidney OMCD cells inflow of urea into the shrunken cell results in restoration of cell volume. In the shrunken peritoneal mesothelial cells, isotonic urea inflow caused a small volume increase and activated regulatory volume decrease (RVD). Isotonic myo-inositol activated RVD in hypertonic medium in both cell types. Isotonic application of both osmolytes caused a sharp increase of intracellular calcium both in peritoneal mesothelial cells and in kidney OMCD principal cells. In conclusion, peritoneal mesothelial cells exhibit RVD mechanisms when challenged with myo-inositol and urea under hyperosmolar isotonic switch from mannitol through involvement of calcium-dependent control. Myo-inositol effects were identical with the ones in OMCD principal cells whereas urea effects in OMCD principal cells led to no RVD induction.

KW - Cell volume regulation

KW - Kidney principal cells

KW - Mesothelial cells

KW - Organic osmolytes

KW - Osmotic stress

UR - http://www.scopus.com/inward/record.url?scp=85116081734&partnerID=8YFLogxK

U2 - 10.3390/biom11101452

DO - 10.3390/biom11101452

M3 - Article

C2 - 34680085

AN - SCOPUS:85116081734

VL - 11

JO - Biomolecules

JF - Biomolecules

SN - 2218-273X

IS - 10

M1 - 1452

ER -

ID: 34349552