TY - JOUR

T1 - Comparison of dried matrix spots and fabric phase sorptive extraction methods for quantification of highly potent analgesic activity agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma using LC–MS/MS

AU - Lastovka, Anastasiya V.

AU - Rogachev, Artem D.

AU - Il'ina, Irina V.

AU - Kabir, Abuzar

AU - Volcho, Konstantin P.

AU - Fadeeva, Valentina P.

AU - Pokrovsky, Andrey G.

AU - Furton, Kenneth G.

AU - Salakhutdinov, Nariman F.

N1 - Copyright © 2019 Elsevier B.V. All rights reserved.

PY - 2019/11/15

Y1 - 2019/11/15

N2 - The methods for quantification of highly potent analgesic agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma were developed and validated using dried matrix spots (DMS) or fabric phase sorptive extraction (FPSE) techniques in combination with LC–MS/MS. 2-Adamantylamine hydrochloride was used as an internal standard (IS). Chromatographic separation was carried out on a reversed-phase column (2.0×75 mm, 5 μm) using water containing 0.1% formic acid and methanol containing 0.1% formic acid as mobile phases in gradient mode at a flow rate of 200 μL/min. The mass spectrometric detection was performed using electrospray ionization (ESI) in positive ion mode. MRM transitions were m/z 284.5 → 137.2/157.4 for the analgesic agent and m/z 152.3 → 93.1/107.2 for IS. Calibration curves were linear within 20–5000 ng/mL in dried plasma spots (DPS) or dried blood spots (DBS) experiments. The linearity was obtained in the range of 20–5000 ng/mL and 50–5000 ng/mL for plasma-FPSE and blood-FPSE experiments, respectively. The intra- and inter-day accuracy and precision did not exceed acceptable limits. The mean extraction recovery (%) was 26 for DPS, 25 for DBS, 38 for plasma-FPSE, 31 for blood-FPSE.

AB - The methods for quantification of highly potent analgesic agent (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol in rat whole blood and plasma were developed and validated using dried matrix spots (DMS) or fabric phase sorptive extraction (FPSE) techniques in combination with LC–MS/MS. 2-Adamantylamine hydrochloride was used as an internal standard (IS). Chromatographic separation was carried out on a reversed-phase column (2.0×75 mm, 5 μm) using water containing 0.1% formic acid and methanol containing 0.1% formic acid as mobile phases in gradient mode at a flow rate of 200 μL/min. The mass spectrometric detection was performed using electrospray ionization (ESI) in positive ion mode. MRM transitions were m/z 284.5 → 137.2/157.4 for the analgesic agent and m/z 152.3 → 93.1/107.2 for IS. Calibration curves were linear within 20–5000 ng/mL in dried plasma spots (DPS) or dried blood spots (DBS) experiments. The linearity was obtained in the range of 20–5000 ng/mL and 50–5000 ng/mL for plasma-FPSE and blood-FPSE experiments, respectively. The intra- and inter-day accuracy and precision did not exceed acceptable limits. The mean extraction recovery (%) was 26 for DPS, 25 for DBS, 38 for plasma-FPSE, 31 for blood-FPSE.

KW - (2R,4aR,7R,8aR)-4,7-dimethyl-2-(thiophen-2-yl)octahydro-2H-chromen-4-ol

KW - Analgesic agent

KW - Dried matrix spots

KW - Fabric phase sorptive extraction

KW - FPSE biofluid sampler

KW - LC–MS/MS

KW - METABOLITES

KW - ASSAY

KW - RAPID-DETERMINATION

KW - LIQUID

KW - VALIDATION

KW - ARRAY DETECTION METHOD

KW - MASS-SPECTROMETRY

KW - LC-MS/MS

KW - DRUGS

KW - AFATINIB

UR - http://www.scopus.com/inward/record.url?scp=85074443097&partnerID=8YFLogxK

U2 - 10.1016/j.jchromb.2019.121813

DO - 10.1016/j.jchromb.2019.121813

M3 - Article

C2 - 31706184

AN - SCOPUS:85074443097

VL - 1132

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

M1 - 121813

ER -