Comparison of Analytical Characteristics of Commercial and in-House Methods for DNA Isolation from Paraffin Histology Blocks. / Oskina, Natalya; Avdalyan, Ashot; Subbotin, Dmitriy et al.
In: Journal of Analytical Oncology, Vol. 6, No. 1, 01.01.2017, p. 1-6.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Comparison of Analytical Characteristics of Commercial and in-House Methods for DNA Isolation from Paraffin Histology Blocks
AU - Oskina, Natalya
AU - Avdalyan, Ashot
AU - Subbotin, Dmitriy
AU - Lazarev, Alexandr
AU - Kel, Alexandr
AU - Kushlinskii, Nikolay
AU - Filipenko, Maxim
PY - 2017/1/1
Y1 - 2017/1/1
N2 - One of the hotspots in clinical research today is molecular genetic analysis of structural DNA alterations. Working with DNA obtained from formalin-fixed and paraffin-embedded (FFPE) tissue specimens is particularly challenging, due to cross-linking and fragmentation of DNA. We performed a comparative analysis of DNA extraction methods from FFPE tissue using two in-house protocols and Qiagen (QIAamp DNA FFPE Tissue Kit) and Roche (High Pure FFPET DNA Isolation Kit) commercial kits presented in the Russian market and used in clinical practice. To assess the quantity and quality of the isolated DNA, we used the real-time PCR to rate DNA yield, the inhibited impurity content and the degree of fragmentation. Our findings may be useful for a medical laboratory that performs testing of somatic mutations for the targeted therapy selection, and researchers who specialized in tumor genome structure studies in respect to patient prognosis and prediction of the sensitivity or resistance of tumor cells to therapy.
AB - One of the hotspots in clinical research today is molecular genetic analysis of structural DNA alterations. Working with DNA obtained from formalin-fixed and paraffin-embedded (FFPE) tissue specimens is particularly challenging, due to cross-linking and fragmentation of DNA. We performed a comparative analysis of DNA extraction methods from FFPE tissue using two in-house protocols and Qiagen (QIAamp DNA FFPE Tissue Kit) and Roche (High Pure FFPET DNA Isolation Kit) commercial kits presented in the Russian market and used in clinical practice. To assess the quantity and quality of the isolated DNA, we used the real-time PCR to rate DNA yield, the inhibited impurity content and the degree of fragmentation. Our findings may be useful for a medical laboratory that performs testing of somatic mutations for the targeted therapy selection, and researchers who specialized in tumor genome structure studies in respect to patient prognosis and prediction of the sensitivity or resistance of tumor cells to therapy.
KW - DNA extraction
KW - Formalin-fixed and paraffin-embedded (FFPE) tissue blocks
KW - High pure FFPET DNA isolation kit
KW - PCR
KW - QIAamp DNA FFPE tissue kit
UR - http://www.scopus.com/inward/record.url?scp=85043559281&partnerID=8YFLogxK
U2 - 10.6000/1927-7229.2017.06.01.1
DO - 10.6000/1927-7229.2017.06.01.1
M3 - Article
VL - 6
SP - 1
EP - 6
JO - Journal of Analytical Oncology
JF - Journal of Analytical Oncology
SN - 1927-7210
IS - 1
ER -
ID: 10454965