TY - JOUR

T1 - Comparative studies of X chromosomes in Cervidae family

AU - Proskuryakova, Anastasia A

AU - Ivanova, Ekaterina S

AU - Makunin, Alexey I

AU - Larkin, Denis M

AU - Ferguson-Smith, Malcolm A

AU - Yang, Fengtang

AU - Uphyrkina, Olga V

AU - Perelman, Polina L

AU - Graphodatsky, Alexander S

N1 - Acknowledgements: The work was supported by a research Grant of the Russian Science Foundation (RSF, 19-14-00034-П). We thank Dmitri Mezentsev and Vasilina Belik from Primorsky Safari-Park (https://safaripark25.ru/) for provided sika deer and reindeer ears biopsy. We would like to sincerely thank Dr. Stephen O’Brien (Laboratory of Genomic Diversity), Dr. Mitchell Bush (Conservation and Research Center, National Zoological Park, Virginia, USA), June Bellizzi and director Richard Hahn (Catoctin wildlife Zoo and Preserve, MD, USA) for kindly providing samples. We would like to thank Mary Tompson (NCI-Frederick, USA) for establishing cell lines. We thank Dr. Anna V. Kukekova and Dr. Jennifer Lynn Johnson (Animal Sciences Department, College of ACES, University of Illinois at Urbana-Champaign, USA) for providing bacterial artifcial chromosome clones. © 2023. The Author(s).

PY - 2023/7/25

Y1 - 2023/7/25

N2 - The family Cervidae is the second most diverse in the infraorder Pecora and is characterized by variability in the diploid chromosome numbers among species. X chromosomes in Cervidae evolved through complex chromosomal rearrangements of conserved segments within the chromosome, changes in centromere position, heterochromatic variation, and X-autosomal translocations. The family Cervidae consists of two subfamilies: Cervinae and Capreolinae. Here we build a detailed X chromosome map with 29 cattle bacterial artificial chromosomes of representatives of both subfamilies: reindeer (Rangifer tarandus), gray brocket deer (Mazama gouazoubira), Chinese water deer (Hydropotes inermis) (Capreolinae); black muntjac (Muntiacus crinifrons), tufted deer (Elaphodus cephalophus), sika deer (Cervus nippon) and red deer (Cervus elaphus) (Cervinae). To track chromosomal rearrangements during Cervidae evolution, we summarized new data, and compared them with available X chromosomal maps and chromosome level assemblies of other species. We demonstrate the types of rearrangements that may have underlined the variability of Cervidae X chromosomes. We detected two types of cervine X chromosome-acrocentric and submetacentric. The acrocentric type is found in three independent deer lineages (subfamily Cervinae and in two Capreolinae tribes-Odocoileini and Capreolini). We show that chromosomal rearrangements on the X-chromosome in Cervidae occur at a higher frequency than in the entire Ruminantia lineage: the rate of rearrangements is 2 per 10 million years.

AB - The family Cervidae is the second most diverse in the infraorder Pecora and is characterized by variability in the diploid chromosome numbers among species. X chromosomes in Cervidae evolved through complex chromosomal rearrangements of conserved segments within the chromosome, changes in centromere position, heterochromatic variation, and X-autosomal translocations. The family Cervidae consists of two subfamilies: Cervinae and Capreolinae. Here we build a detailed X chromosome map with 29 cattle bacterial artificial chromosomes of representatives of both subfamilies: reindeer (Rangifer tarandus), gray brocket deer (Mazama gouazoubira), Chinese water deer (Hydropotes inermis) (Capreolinae); black muntjac (Muntiacus crinifrons), tufted deer (Elaphodus cephalophus), sika deer (Cervus nippon) and red deer (Cervus elaphus) (Cervinae). To track chromosomal rearrangements during Cervidae evolution, we summarized new data, and compared them with available X chromosomal maps and chromosome level assemblies of other species. We demonstrate the types of rearrangements that may have underlined the variability of Cervidae X chromosomes. We detected two types of cervine X chromosome-acrocentric and submetacentric. The acrocentric type is found in three independent deer lineages (subfamily Cervinae and in two Capreolinae tribes-Odocoileini and Capreolini). We show that chromosomal rearrangements on the X-chromosome in Cervidae occur at a higher frequency than in the entire Ruminantia lineage: the rate of rearrangements is 2 per 10 million years.

KW - Cattle

KW - Animals

KW - Deer/genetics

KW - Ruminants/genetics

KW - Chromosomes

KW - Muntjacs/genetics

KW - X Chromosome/genetics

KW - Reindeer/genetics

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85165668949&origin=inward&txGid=4f975c15b54cab545b1beb696f09e9b8

U2 - 10.1038/s41598-023-39088-4

DO - 10.1038/s41598-023-39088-4

M3 - Article

C2 - 37491593

VL - 13

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 11992

ER -