Research output: Contribution to journal › Article › peer-review
Comparative Analysis of Nucleotide Fluorescent Analogs for Registration of DNA Conformational Changes Induced by Interaction with Formamidopyrimidine-DNA Glycosylase Fpg. / Kuznetsova, A. A.; Kladova, O. A.; Barthes, Nicolas P.F. et al.
In: Russian Journal of Bioorganic Chemistry, Vol. 45, No. 6, 01.11.2019, p. 591-598.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Comparative Analysis of Nucleotide Fluorescent Analogs for Registration of DNA Conformational Changes Induced by Interaction with Formamidopyrimidine-DNA Glycosylase Fpg
AU - Kuznetsova, A. A.
AU - Kladova, O. A.
AU - Barthes, Nicolas P.F.
AU - Michel, Benoit Y.
AU - Burger, Alain
AU - Fedorova, O. S.
AU - Kuznetsov, N. A.
PY - 2019/11/1
Y1 - 2019/11/1
N2 - DNA-substrates containing fluorescent DNA base analogs are widely used to study protein–nucleic acid interactions. In the case of DNA-recognizing enzymes, this approach allows one to register conformational changes in DNA during the formation of enzyme–substrate complexes. An important part of such research is the design of model DNA substrates, which includes both the photophysical properties of the fluorescent groups and their location relative to a specific recognition site, namely, in the same chain on the 5′-, 3′-side or in the complementary chain opposite the specific site. In this work, we report a comparative study of the sensitivity of various fluorescent DNA base analogs, such as 2-aminopurine (aPu), pyrrolocytosine (CPy), 1,3-diaza-2-oxophenoxazine (tCO) and 3-hydroxychromone (3HC), to conformational transformations of DNA in the process of interaction with formamidopyrimidine-DNA glycosylase (Fpg) from Escherichia coli.
AB - DNA-substrates containing fluorescent DNA base analogs are widely used to study protein–nucleic acid interactions. In the case of DNA-recognizing enzymes, this approach allows one to register conformational changes in DNA during the formation of enzyme–substrate complexes. An important part of such research is the design of model DNA substrates, which includes both the photophysical properties of the fluorescent groups and their location relative to a specific recognition site, namely, in the same chain on the 5′-, 3′-side or in the complementary chain opposite the specific site. In this work, we report a comparative study of the sensitivity of various fluorescent DNA base analogs, such as 2-aminopurine (aPu), pyrrolocytosine (CPy), 1,3-diaza-2-oxophenoxazine (tCO) and 3-hydroxychromone (3HC), to conformational transformations of DNA in the process of interaction with formamidopyrimidine-DNA glycosylase (Fpg) from Escherichia coli.
KW - conformational dynamics
KW - DNA glycosylase Fpg
KW - fluorescence
KW - protein–nucleic acid interactions
UR - http://www.scopus.com/inward/record.url?scp=85078610382&partnerID=8YFLogxK
U2 - 10.1134/S1068162019060256
DO - 10.1134/S1068162019060256
M3 - Article
AN - SCOPUS:85078610382
VL - 45
SP - 591
EP - 598
JO - Russian Journal of Bioorganic Chemistry
JF - Russian Journal of Bioorganic Chemistry
SN - 1068-1620
IS - 6
ER -
ID: 23287378