Research output: Contribution to journal › Article › peer-review
Combined effects of oncolytic vaccinia virus and dendritic cells on the progression of melanoma B16-F10 in mice. / Goncharova, Elena P.; Gamburg, Tatiana A.; Markov, Oleg V. et al.
In: Journal of Cancer Metastasis and Treatment, Vol. 8, 10, 2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Combined effects of oncolytic vaccinia virus and dendritic cells on the progression of melanoma B16-F10 in mice
AU - Goncharova, Elena P.
AU - Gamburg, Tatiana A.
AU - Markov, Oleg V.
AU - Zenkova, Marina A.
N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research project # 18-34-20109, Russian state budget of ICBFM SB RAS project # 121031300044-5, Russian Science Foundation RSF # 19-74-30011. Publisher Copyright: © The Author(s) 2022.
PY - 2022
Y1 - 2022
N2 - Aim: We aimed to test the hypothesis that loading of dendritic cells (DCs) with both viral and tumor-specific antigens would enhance the efficacy antitumor DC-based therapy applied simultaneously with oncolytic virus. Methods: Vaccinia virus LIVP/GFP and melanoma B16-F10 were used in this study. DCs were pulsed with various combinations of viral and tumor-associated antigens. The maturation status of DCs was verified by expression of the markers CD80, CD86, and CCR7 and assessment of IL-6, TNF-α, and IL-12 secretion. The most efficient combination of antigens for DC loading was selected based on the analysis of the cytotoxic activity of T lymphocytes. Combination therapy using vaccinia virus LIVP/GFP and DCs pulsed with viral and tumor-specific antigens was administered to the B16-F10 melanoma/mouse C57Bl tumor model. Results: We found that loading of DCs with viral antigens, or with a combination of viral and tumor antigens, resulted in similar levels of expression of DC maturation markers. The maximal in vitro cytotoxicity against virus-infected and non-infected B16 melanoma cells exhibited T lymphocytes activated by DCs loaded with the heat inactivated lysate of vaccinia virus LIVP/GFP infected tumor cell. The results show that the combination of vaccinia virus LIVP/GFP and DCs loaded with both tumor and viral antigens inhibit tumor growth of B16-F10 murine melanoma by more than two-fold. Conclusions: Combination therapy with oncolytic vaccinia virus LIVP/GFP and tumor/virus antigen-loaded DCs limited the growth of established melanoma B16-F10, but no synergistic antitumor effects were observed. We propose that optimization of the therapy regimen could enhance the efficiency of combination therapy.
AB - Aim: We aimed to test the hypothesis that loading of dendritic cells (DCs) with both viral and tumor-specific antigens would enhance the efficacy antitumor DC-based therapy applied simultaneously with oncolytic virus. Methods: Vaccinia virus LIVP/GFP and melanoma B16-F10 were used in this study. DCs were pulsed with various combinations of viral and tumor-associated antigens. The maturation status of DCs was verified by expression of the markers CD80, CD86, and CCR7 and assessment of IL-6, TNF-α, and IL-12 secretion. The most efficient combination of antigens for DC loading was selected based on the analysis of the cytotoxic activity of T lymphocytes. Combination therapy using vaccinia virus LIVP/GFP and DCs pulsed with viral and tumor-specific antigens was administered to the B16-F10 melanoma/mouse C57Bl tumor model. Results: We found that loading of DCs with viral antigens, or with a combination of viral and tumor antigens, resulted in similar levels of expression of DC maturation markers. The maximal in vitro cytotoxicity against virus-infected and non-infected B16 melanoma cells exhibited T lymphocytes activated by DCs loaded with the heat inactivated lysate of vaccinia virus LIVP/GFP infected tumor cell. The results show that the combination of vaccinia virus LIVP/GFP and DCs loaded with both tumor and viral antigens inhibit tumor growth of B16-F10 murine melanoma by more than two-fold. Conclusions: Combination therapy with oncolytic vaccinia virus LIVP/GFP and tumor/virus antigen-loaded DCs limited the growth of established melanoma B16-F10, but no synergistic antitumor effects were observed. We propose that optimization of the therapy regimen could enhance the efficiency of combination therapy.
KW - cancer immunotherapy
KW - cytotoxic T lymphocytes
KW - dendritic cells
KW - melanoma B16
KW - Oncolytic virus
KW - vaccinia virus
UR - http://www.scopus.com/inward/record.url?scp=85136273673&partnerID=8YFLogxK
U2 - 10.20517/2394-4722.2021.195
DO - 10.20517/2394-4722.2021.195
M3 - Article
AN - SCOPUS:85136273673
VL - 8
JO - Journal of Cancer Metastasis and Treatment
JF - Journal of Cancer Metastasis and Treatment
SN - 2394-4722
M1 - 10
ER -
ID: 36957982