Clustering of Stearic Acids in Model Phospholipid Membranes Revealed by Double Electron-Electron Resonance

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Clustering of Stearic Acids in Model Phospholipid Membranes Revealed by Double Electron-Electron Resonance. / Smorygina, Anna S.; Golysheva, Elena A.; Dzuba, Sergei A.

In: Langmuir, Vol. 37, No. 47, 30.11.2021, p. 13909-13916.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{88d09d4048294db08daa008b3f99c9fe,

title = "Clustering of Stearic Acids in Model Phospholipid Membranes Revealed by Double Electron-Electron Resonance",

abstract = "Free fatty acids play various important roles in biological membranes. Double electron-electron resonance spectroscopy (DEER, also known as PELDOR) of spin-labeled biomolecules is capable of studying magnetic dipole-dipole (d-d) interactions between spin labels at the nanoscale range of distances. Here, DEER is applied to study intermolecular d-d interactions between doxyl-spin-labeled stearic acids (DSA) in gel-phase phospholipid bilayers composed either of an equimolecular mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine or of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. DEER data obtained for different DSA concentrations showed that DSA molecules at their concentration in the bilayer χ larger than 0.5 mol % are assembled into lateral lipid-mediated clusters, with a characteristic intermolecular distance of 2 nm. Some evidences were obtained indicating that clusters may consist of {"}subclusters{"}, alternatively appearing in two opposite leaflets. Conventional electron paramagnetic resonance (EPR) spectra for the gel-phase bilayers showed that for χ larger than 2 mol % the molecules in the clusters stick together, forming oligomers. Room-temperature EPR spectra for the liquid-crystalline phase were found to change noticeably for χ larger than 0.5 mol %, which may indicate the clustering in a liquid-crystalline phase similar to that observed by DEER in the gel phase. ",

author = "Smorygina, {Anna S.} and Golysheva, {Elena A.} and Dzuba, {Sergei A.}",

note = "Funding Information: This work was supported by the Russian Science Foundation, project # 21-13-00025. Publisher Copyright: {\textcopyright} 2021 American Chemical Society.",

year = "2021",

month = nov,

day = "30",

doi = "10.1021/acs.langmuir.1c02460",

language = "English",

volume = "37",

pages = "13909--13916",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American Chemical Society",

number = "47",

}

RIS

TY - JOUR

T1 - Clustering of Stearic Acids in Model Phospholipid Membranes Revealed by Double Electron-Electron Resonance

AU - Smorygina, Anna S.

AU - Golysheva, Elena A.

AU - Dzuba, Sergei A.

PY - 2021/11/30

Y1 - 2021/11/30

N2 - Free fatty acids play various important roles in biological membranes. Double electron-electron resonance spectroscopy (DEER, also known as PELDOR) of spin-labeled biomolecules is capable of studying magnetic dipole-dipole (d-d) interactions between spin labels at the nanoscale range of distances. Here, DEER is applied to study intermolecular d-d interactions between doxyl-spin-labeled stearic acids (DSA) in gel-phase phospholipid bilayers composed either of an equimolecular mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine or of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. DEER data obtained for different DSA concentrations showed that DSA molecules at their concentration in the bilayer χ larger than 0.5 mol % are assembled into lateral lipid-mediated clusters, with a characteristic intermolecular distance of 2 nm. Some evidences were obtained indicating that clusters may consist of "subclusters", alternatively appearing in two opposite leaflets. Conventional electron paramagnetic resonance (EPR) spectra for the gel-phase bilayers showed that for χ larger than 2 mol % the molecules in the clusters stick together, forming oligomers. Room-temperature EPR spectra for the liquid-crystalline phase were found to change noticeably for χ larger than 0.5 mol %, which may indicate the clustering in a liquid-crystalline phase similar to that observed by DEER in the gel phase.

AB - Free fatty acids play various important roles in biological membranes. Double electron-electron resonance spectroscopy (DEER, also known as PELDOR) of spin-labeled biomolecules is capable of studying magnetic dipole-dipole (d-d) interactions between spin labels at the nanoscale range of distances. Here, DEER is applied to study intermolecular d-d interactions between doxyl-spin-labeled stearic acids (DSA) in gel-phase phospholipid bilayers composed either of an equimolecular mixture of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine and 1,2-dioleoyl-sn-glycero-3-phosphocholine or of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine. DEER data obtained for different DSA concentrations showed that DSA molecules at their concentration in the bilayer χ larger than 0.5 mol % are assembled into lateral lipid-mediated clusters, with a characteristic intermolecular distance of 2 nm. Some evidences were obtained indicating that clusters may consist of "subclusters", alternatively appearing in two opposite leaflets. Conventional electron paramagnetic resonance (EPR) spectra for the gel-phase bilayers showed that for χ larger than 2 mol % the molecules in the clusters stick together, forming oligomers. Room-temperature EPR spectra for the liquid-crystalline phase were found to change noticeably for χ larger than 0.5 mol %, which may indicate the clustering in a liquid-crystalline phase similar to that observed by DEER in the gel phase.

UR - http://www.scopus.com/inward/record.url?scp=85120050993&partnerID=8YFLogxK

U2 - 10.1021/acs.langmuir.1c02460

DO - 10.1021/acs.langmuir.1c02460

M3 - Article

C2 - 34787421

AN - SCOPUS:85120050993

VL - 37

SP - 13909

EP - 13916

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 47

ER -

ID: 34856940