TY - JOUR

T1 - Chylomicrons against light scattering: The battle for characterization

AU - Chernova, Darya N.

AU - Konokhova, Anastasiya I.

AU - Novikova, Olga A.

AU - Yurkin, Maxim A.

AU - Strokotov, Dmitry I.

AU - Karpenko, Andrei A.

AU - Chernyshev, Andrei V.

AU - Maltsev, Valeri P.

N1 - Funding Information: This work was supported by (grant no. 17-75-20117). Funding Information: information Russian Science Foundation, Grant/Award Number: 17-75-20117This work was supported by Russian Science Foundation (grant no. 17-75-20117). Publisher Copyright: © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Chylomicrons (CMs) are lipoprotein particles circulating in blood and transporting dietary lipids. Optically speaking, CMs are small compared to the wavelength of visible light and widely distributed by the size and refractive index (RI). Consequently, intensity of light scattered by the CMs scales with up to the sixth power of their size, hampering simultaneous analysis of 60 and 600 nm CMs. We present an accurate method for quantitative characterization of large-size CM subpopulation by the distributions over size and RI. For the first time the CM characteristics have been determined at a single particle level based on angle-resolved light-scattering measurements. We applied the developed method to 2 key processes relating to CM metabolism, namely in vivo dynamics of CMs in blood plasma after a meal and in vitro lipolysis of CMs by the lipoprotein lipase in postheparin plasma. We have observed the substantial variations in CM concentration, size and RI distributions. This opens the way for a multitude of medical applications involving screening of CM metabolism, which we exemplified by revealing large differences in CM characteristics after a 12-hour fast between a healthy volunteer and a patient with atherosclerosis.

AB - Chylomicrons (CMs) are lipoprotein particles circulating in blood and transporting dietary lipids. Optically speaking, CMs are small compared to the wavelength of visible light and widely distributed by the size and refractive index (RI). Consequently, intensity of light scattered by the CMs scales with up to the sixth power of their size, hampering simultaneous analysis of 60 and 600 nm CMs. We present an accurate method for quantitative characterization of large-size CM subpopulation by the distributions over size and RI. For the first time the CM characteristics have been determined at a single particle level based on angle-resolved light-scattering measurements. We applied the developed method to 2 key processes relating to CM metabolism, namely in vivo dynamics of CMs in blood plasma after a meal and in vitro lipolysis of CMs by the lipoprotein lipase in postheparin plasma. We have observed the substantial variations in CM concentration, size and RI distributions. This opens the way for a multitude of medical applications involving screening of CM metabolism, which we exemplified by revealing large differences in CM characteristics after a 12-hour fast between a healthy volunteer and a patient with atherosclerosis.

KW - characterization

KW - chylomicrons

KW - flow cytometry

KW - light scattering

KW - lipolysis

KW - OBESE MEN

KW - ATHEROSCLEROSIS

KW - TRIGLYCERIDES

KW - SCANNING FLOW-CYTOMETRY

KW - APOLIPOPROTEIN B-48

KW - SIZE DISTRIBUTIONS

KW - METABOLISM

KW - PLASMA-LIPOPROTEINS

KW - HUMAN LYMPH

KW - PARTICLE-SIZE

KW - Humans

KW - Case-Control Studies

KW - Lipolysis

KW - Chylomicrons/blood

KW - Light

KW - Postprandial Period

KW - Scattering, Radiation

KW - Atherosclerosis/blood

UR - http://www.scopus.com/inward/record.url?scp=85054483586&partnerID=8YFLogxK

U2 - 10.1002/jbio.201700381

DO - 10.1002/jbio.201700381

M3 - Article

C2 - 29603652

AN - SCOPUS:85054483586

VL - 11

JO - Journal of Biophotonics

JF - Journal of Biophotonics

SN - 1864-063X

IS - 10

M1 - 201700381

ER -