Research output: Contribution to journal › Article › peer-review
Chromosome painting does not support a sex chromosome turnover in lacerta agilis linnaeus, 1758. / Lisachov, Artem P.; Giovannotti, Massimo; Pereira, Jorge C. et al.
In: Cytogenetic and Genome Research, Vol. 160, No. 3, 01.04.2020, p. 134-140.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Chromosome painting does not support a sex chromosome turnover in lacerta agilis linnaeus, 1758
AU - Lisachov, Artem P.
AU - Giovannotti, Massimo
AU - Pereira, Jorge C.
AU - Andreyushkova, Daria A.
AU - Romanenko, Svetlana A.
AU - Ferguson-Smith, Malcolm A.
AU - Borodin, Pavel M.
AU - Trifonov, Vladimir A.
N1 - Publisher Copyright: © 2020 S. Karger AG, Basel.
PY - 2020/4/1
Y1 - 2020/4/1
N2 - Reptiles show a remarkable diversity of sex determination mechanisms and sex chromosome systems, derived from different autosomal pairs. The origin of the ZW sex chromosomes of Lacerta agilis, a widespread Eurasian lizard species, is a matter of discussion: Is it a small macrochromosome from the 11-18 group common to all lacertids, or does this species have a unique ZW pair derived from the large chromosome 5? Using independent molecular cytogenetic methods, we investigated the karyotype of L. agilis exigua from Siberia, Russia, to identify the sex chromosomes. FISH with a flow-sorted chromosome painting probe derived from L. strigata and specific to chromosomes 13, 14, and Z confirmed that the Z chromosome of L. agilis is a small macrochromosome, the same as in L. strigata. FISH with the telomeric probe showed an extensive accumulation of the telomere-like repeat in the W chromosome in agreement with previous studies, excluding the possibility that the lineages of L. agilis studied in different works could have different sex chromosome systems due to a putative intra-species polymorphism. Our results reinforce the idea of the stability of the sex chromosomes and lack of evidence for sex-chromosome turnovers in known species of Lacertidae.
AB - Reptiles show a remarkable diversity of sex determination mechanisms and sex chromosome systems, derived from different autosomal pairs. The origin of the ZW sex chromosomes of Lacerta agilis, a widespread Eurasian lizard species, is a matter of discussion: Is it a small macrochromosome from the 11-18 group common to all lacertids, or does this species have a unique ZW pair derived from the large chromosome 5? Using independent molecular cytogenetic methods, we investigated the karyotype of L. agilis exigua from Siberia, Russia, to identify the sex chromosomes. FISH with a flow-sorted chromosome painting probe derived from L. strigata and specific to chromosomes 13, 14, and Z confirmed that the Z chromosome of L. agilis is a small macrochromosome, the same as in L. strigata. FISH with the telomeric probe showed an extensive accumulation of the telomere-like repeat in the W chromosome in agreement with previous studies, excluding the possibility that the lineages of L. agilis studied in different works could have different sex chromosome systems due to a putative intra-species polymorphism. Our results reinforce the idea of the stability of the sex chromosomes and lack of evidence for sex-chromosome turnovers in known species of Lacertidae.
KW - Lacertidae
KW - Reptilia
KW - Sex chromosomes
KW - Sex determination
KW - Zoo-fish
KW - KARYOTYPES
KW - REPTILIA
KW - LIZARD
KW - Zoo-FISH
KW - DETERMINING MECHANISMS
KW - LACERTA-AGILIS
KW - GENOME
KW - DIFFERENTIATION
KW - BIRDS
UR - http://www.scopus.com/inward/record.url?scp=85082424734&partnerID=8YFLogxK
U2 - 10.1159/000506321
DO - 10.1159/000506321
M3 - Article
C2 - 32092753
AN - SCOPUS:85082424734
VL - 160
SP - 134
EP - 140
JO - Cytogenetic and Genome Research
JF - Cytogenetic and Genome Research
SN - 1424-8581
IS - 3
ER -
ID: 23907820