Research output: Contribution to journal › Article › peer-review
Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line. / Lukyanchikova, Varvara; Nuriddinov, Miroslav; Khabarova, Anna et al.
In: Scientific Data, Vol. 12, No. 1, 26.09.2025, p. 1566.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line
AU - Lukyanchikova, Varvara
AU - Nuriddinov, Miroslav
AU - Khabarova, Anna
AU - Gridina, Maria
AU - Popov, Andrey
AU - Belokopytova, Polina
AU - Sharakhov, Igor V
AU - Fishman, Veniamin
N1 - This work was supported by the grant of the state program of the «Sirius» Federal Territory «Scientific and technological development of the «Sirius» Federal Territory» (Agreement №26-03, 27/09/2024) (Hi-C libraries preparation, data analysis, manuscript preparation). An. stephensi MSQ43 cell line was initially obtained through Malaria Research and Reference Reagent Resource Center (MR4) stocks and BEI Resources, NIAID, NIH. All computations were performed using nodes of the high-throughput cluster of the Novosibirsk State University (supported by the Ministry of Science and Higher Education of the Russian Federation, grant #FSUS-2024-0018), and bioinformatics resource center of the Institute of Cytology and Genetics (state project FWNR-2022-0019). We acknowledge the Center for Shared Use of Flow Cytometry SB RAS for providing FACS sorting facilities.
PY - 2025/9/26
Y1 - 2025/9/26
N2 - Anopheles mosquitoes are known as dominant vectors of malaria parasites and other viral and bacterial pathogens. Deciphering Anopheles genomes has opened a new era of research and allowed in-depth investigation of several molecular mechanisms involved in pathogen transmission. However, there is a lack of appropriate model systems to study specific vector-pathogen interactions at the molecular level. Even inbred mosquito colonies represent genetically heterogeneous population and require special care facilities. Insect cell cultures could serve as a useful alternative due to the ease of handling and maintenance, especially for genomic studies requiring millions of cells per experiment. Here, we provide chromatin contact maps and a dataset of epigenetic characteristics, including histone mark profiles and RNA-seq data, as well as transfection conditions and promoter specificity for the mosquito cell line MSQ43.
AB - Anopheles mosquitoes are known as dominant vectors of malaria parasites and other viral and bacterial pathogens. Deciphering Anopheles genomes has opened a new era of research and allowed in-depth investigation of several molecular mechanisms involved in pathogen transmission. However, there is a lack of appropriate model systems to study specific vector-pathogen interactions at the molecular level. Even inbred mosquito colonies represent genetically heterogeneous population and require special care facilities. Insect cell cultures could serve as a useful alternative due to the ease of handling and maintenance, especially for genomic studies requiring millions of cells per experiment. Here, we provide chromatin contact maps and a dataset of epigenetic characteristics, including histone mark profiles and RNA-seq data, as well as transfection conditions and promoter specificity for the mosquito cell line MSQ43.
KW - Anopheles/genetics
KW - Animals
KW - Chromatin/genetics
KW - Cell Line
KW - Transcriptome
KW - Chromatin Immunoprecipitation Sequencing
KW - Mosquito Vectors/genetics
KW - Epigenesis, Genetic
UR - https://pubmed.ncbi.nlm.nih.gov/41006309/
UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105017417220&origin=inward
UR - https://pmc.ncbi.nlm.nih.gov/articles/PMC12474893/
U2 - 10.1038/s41597-025-05721-2
DO - 10.1038/s41597-025-05721-2
M3 - Article
C2 - 41006309
VL - 12
SP - 1566
JO - Scientific Data
JF - Scientific Data
SN - 2052-4463
IS - 1
ER -
ID: 70304179