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Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line. / Lukyanchikova, Varvara; Nuriddinov, Miroslav; Khabarova, Anna et al.

In: Scientific Data, Vol. 12, No. 1, 26.09.2025, p. 1566.

Research output: Contribution to journalArticlepeer-review

Harvard

Lukyanchikova, V, Nuriddinov, M, Khabarova, A, Gridina, M, Popov, A, Belokopytova, P, Sharakhov, IV & Fishman, V 2025, 'Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line', Scientific Data, vol. 12, no. 1, pp. 1566. https://doi.org/10.1038/s41597-025-05721-2

APA

Lukyanchikova, V., Nuriddinov, M., Khabarova, A., Gridina, M., Popov, A., Belokopytova, P., Sharakhov, I. V., & Fishman, V. (2025). Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line. Scientific Data, 12(1), 1566. https://doi.org/10.1038/s41597-025-05721-2

Vancouver

Lukyanchikova V, Nuriddinov M, Khabarova A, Gridina M, Popov A, Belokopytova P et al. Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line. Scientific Data. 2025 Sept 26;12(1):1566. doi: 10.1038/s41597-025-05721-2

Author

Lukyanchikova, Varvara ; Nuriddinov, Miroslav ; Khabarova, Anna et al. / Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line. In: Scientific Data. 2025 ; Vol. 12, No. 1. pp. 1566.

BibTeX

@article{86ea45a2d9aa49c58161c7e18bb22adb,
title = "Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line",
abstract = "Anopheles mosquitoes are known as dominant vectors of malaria parasites and other viral and bacterial pathogens. Deciphering Anopheles genomes has opened a new era of research and allowed in-depth investigation of several molecular mechanisms involved in pathogen transmission. However, there is a lack of appropriate model systems to study specific vector-pathogen interactions at the molecular level. Even inbred mosquito colonies represent genetically heterogeneous population and require special care facilities. Insect cell cultures could serve as a useful alternative due to the ease of handling and maintenance, especially for genomic studies requiring millions of cells per experiment. Here, we provide chromatin contact maps and a dataset of epigenetic characteristics, including histone mark profiles and RNA-seq data, as well as transfection conditions and promoter specificity for the mosquito cell line MSQ43.",
keywords = "Anopheles/genetics, Animals, Chromatin/genetics, Cell Line, Transcriptome, Chromatin Immunoprecipitation Sequencing, Mosquito Vectors/genetics, Epigenesis, Genetic",
author = "Varvara Lukyanchikova and Miroslav Nuriddinov and Anna Khabarova and Maria Gridina and Andrey Popov and Polina Belokopytova and Sharakhov, {Igor V} and Veniamin Fishman",
note = "This work was supported by the grant of the state program of the «Sirius» Federal Territory «Scientific and technological development of the «Sirius» Federal Territory» (Agreement №26-03, 27/09/2024) (Hi-C libraries preparation, data analysis, manuscript preparation). An. stephensi MSQ43 cell line was initially obtained through Malaria Research and Reference Reagent Resource Center (MR4) stocks and BEI Resources, NIAID, NIH. All computations were performed using nodes of the high-throughput cluster of the Novosibirsk State University (supported by the Ministry of Science and Higher Education of the Russian Federation, grant #FSUS-2024-0018), and bioinformatics resource center of the Institute of Cytology and Genetics (state project FWNR-2022-0019). We acknowledge the Center for Shared Use of Flow Cytometry SB RAS for providing FACS sorting facilities. ",
year = "2025",
month = sep,
day = "26",
doi = "10.1038/s41597-025-05721-2",
language = "English",
volume = "12",
pages = "1566",
journal = "Scientific Data",
issn = "2052-4463",
publisher = "Nature Publishing Group",
number = "1",

}

RIS

TY - JOUR

T1 - Chromatin landscape, transcriptomic and ChIP-seq profiling of Anopheles stephensi MSQ43 cell line

AU - Lukyanchikova, Varvara

AU - Nuriddinov, Miroslav

AU - Khabarova, Anna

AU - Gridina, Maria

AU - Popov, Andrey

AU - Belokopytova, Polina

AU - Sharakhov, Igor V

AU - Fishman, Veniamin

N1 - This work was supported by the grant of the state program of the «Sirius» Federal Territory «Scientific and technological development of the «Sirius» Federal Territory» (Agreement №26-03, 27/09/2024) (Hi-C libraries preparation, data analysis, manuscript preparation). An. stephensi MSQ43 cell line was initially obtained through Malaria Research and Reference Reagent Resource Center (MR4) stocks and BEI Resources, NIAID, NIH. All computations were performed using nodes of the high-throughput cluster of the Novosibirsk State University (supported by the Ministry of Science and Higher Education of the Russian Federation, grant #FSUS-2024-0018), and bioinformatics resource center of the Institute of Cytology and Genetics (state project FWNR-2022-0019). We acknowledge the Center for Shared Use of Flow Cytometry SB RAS for providing FACS sorting facilities.

PY - 2025/9/26

Y1 - 2025/9/26

N2 - Anopheles mosquitoes are known as dominant vectors of malaria parasites and other viral and bacterial pathogens. Deciphering Anopheles genomes has opened a new era of research and allowed in-depth investigation of several molecular mechanisms involved in pathogen transmission. However, there is a lack of appropriate model systems to study specific vector-pathogen interactions at the molecular level. Even inbred mosquito colonies represent genetically heterogeneous population and require special care facilities. Insect cell cultures could serve as a useful alternative due to the ease of handling and maintenance, especially for genomic studies requiring millions of cells per experiment. Here, we provide chromatin contact maps and a dataset of epigenetic characteristics, including histone mark profiles and RNA-seq data, as well as transfection conditions and promoter specificity for the mosquito cell line MSQ43.

AB - Anopheles mosquitoes are known as dominant vectors of malaria parasites and other viral and bacterial pathogens. Deciphering Anopheles genomes has opened a new era of research and allowed in-depth investigation of several molecular mechanisms involved in pathogen transmission. However, there is a lack of appropriate model systems to study specific vector-pathogen interactions at the molecular level. Even inbred mosquito colonies represent genetically heterogeneous population and require special care facilities. Insect cell cultures could serve as a useful alternative due to the ease of handling and maintenance, especially for genomic studies requiring millions of cells per experiment. Here, we provide chromatin contact maps and a dataset of epigenetic characteristics, including histone mark profiles and RNA-seq data, as well as transfection conditions and promoter specificity for the mosquito cell line MSQ43.

KW - Anopheles/genetics

KW - Animals

KW - Chromatin/genetics

KW - Cell Line

KW - Transcriptome

KW - Chromatin Immunoprecipitation Sequencing

KW - Mosquito Vectors/genetics

KW - Epigenesis, Genetic

UR - https://pubmed.ncbi.nlm.nih.gov/41006309/

UR - https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=105017417220&origin=inward

UR - https://pmc.ncbi.nlm.nih.gov/articles/PMC12474893/

U2 - 10.1038/s41597-025-05721-2

DO - 10.1038/s41597-025-05721-2

M3 - Article

C2 - 41006309

VL - 12

SP - 1566

JO - Scientific Data

JF - Scientific Data

SN - 2052-4463

IS - 1

ER -

ID: 70304179