Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes. / Aulova, Kseniya S.; Urusov, Andrey E.; Toporkova, Ludmila B. et al.
In: Molecules, Vol. 27, No. 7, 2195, 01.04.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Cell Differentiation and Proliferation in the Bone Marrow and Other Organs of 2D2 Mice during Spontaneous Development of EAE Leading to the Production of Abzymes
AU - Aulova, Kseniya S.
AU - Urusov, Andrey E.
AU - Toporkova, Ludmila B.
AU - Sedykh, Sergey E.
AU - Shevchenko, Juliya A.
AU - Tereshchenko, Valeriy P.
AU - Sennikov, Sergei
AU - Orlovskaya, Irina A.
AU - Nevinsky, Georgy A.
N1 - This research was endorsed by a grant of the Russian Foundation for Basic Research (20-04-00281) and the Russian State-funded budget project of ICBFM SB RAS 0245-2021-0009 (121031300041-4).
PY - 2022/4/1
Y1 - 2022/4/1
N2 - The exact cellular and molecular mechanisms of multiple sclerosis and other autoimmune diseases have not been established. Autoimmune pathologies are known to be associated with faults in the immune system and changes in the differentiation profiles of bone marrow stem cells. This study analyzed various characteristics of experimental autoimmune encephalomyelitis (EAE) in 2D2 mice. Differentiation profiles of six hematopoietic stem cells of bone marrow were found to significantly differ in 2D2 male and female mice during the spontaneous development of EAE. In addition, we found various properties of B and T cells, CD4+ and CD8+ lymphocytes in blood and several organs (bone marrow, spleen, thymus, and lymph nodes) of 2D2 male and female mice to be considerably different. These changes in hematopoietic stem cells differentiation profiles and level of lymphocyte proliferation in various organs of 2D2 mice were found to induce the production of IgGs against DNA, myelin basic protein, and myelin oligodendrocyte glycoprotein, increasing the number of autoantibodies hydrolyzing these substrates. We compared the changes of these immunological and biochemical parameters in 2D2 mice with those of mice of two other lines (Th and C57BL/6), also prone to spontaneous development of EAE. Some noticeable and even extreme variations were found in the time-related development of parameters between male and female mice of 2D2, Th, and C57BL/6 lines. Despite some differences, mice of all three lines demonstrated the changes in hematopoietic stem cells profiles, lymphocyte content, and production of catalytic autoantibodies. Given that these changes are harmful to mice, we believe them to cause the development of experimental autoimmune encephalomyelitis.
AB - The exact cellular and molecular mechanisms of multiple sclerosis and other autoimmune diseases have not been established. Autoimmune pathologies are known to be associated with faults in the immune system and changes in the differentiation profiles of bone marrow stem cells. This study analyzed various characteristics of experimental autoimmune encephalomyelitis (EAE) in 2D2 mice. Differentiation profiles of six hematopoietic stem cells of bone marrow were found to significantly differ in 2D2 male and female mice during the spontaneous development of EAE. In addition, we found various properties of B and T cells, CD4+ and CD8+ lymphocytes in blood and several organs (bone marrow, spleen, thymus, and lymph nodes) of 2D2 male and female mice to be considerably different. These changes in hematopoietic stem cells differentiation profiles and level of lymphocyte proliferation in various organs of 2D2 mice were found to induce the production of IgGs against DNA, myelin basic protein, and myelin oligodendrocyte glycoprotein, increasing the number of autoantibodies hydrolyzing these substrates. We compared the changes of these immunological and biochemical parameters in 2D2 mice with those of mice of two other lines (Th and C57BL/6), also prone to spontaneous development of EAE. Some noticeable and even extreme variations were found in the time-related development of parameters between male and female mice of 2D2, Th, and C57BL/6 lines. Despite some differences, mice of all three lines demonstrated the changes in hematopoietic stem cells profiles, lymphocyte content, and production of catalytic autoantibodies. Given that these changes are harmful to mice, we believe them to cause the development of experimental autoimmune encephalomyelitis.
KW - Th and C57BL
KW - 6 EAE mice
KW - development of experimental autoimmune encephalomyelitis (EAE)
KW - hematopoietic stem cells differentiation
KW - lymphocyte proliferation in different organs
KW - catalytic antibodies
KW - MYELIN BASIC-PROTEIN
KW - MULTIPLE-SCLEROSIS
KW - HYDROLYZING ANTIBODIES
KW - CATALYTIC ANTIBODIES
KW - COLONY FORMATION
KW - MOUSE MODELS
KW - B-CELLS
KW - AUTOIMMUNE
KW - SERA
KW - DNA
KW - 2D2
KW - and C57BL/6 EAE mice
KW - Th
KW - Bone Marrow/pathology
KW - Peptide Fragments
KW - Cell Proliferation
KW - Male
KW - Female
KW - Cell Differentiation
KW - Autoantibodies
KW - Encephalomyelitis, Autoimmune, Experimental/pathology
KW - Mice, Inbred C57BL
KW - Myelin-Oligodendrocyte Glycoprotein
KW - Animals
KW - Antibodies, Catalytic
KW - Mice
UR - http://www.scopus.com/inward/record.url?scp=85127886709&partnerID=8YFLogxK
U2 - 10.3390/molecules27072195
DO - 10.3390/molecules27072195
M3 - Article
C2 - 35408594
VL - 27
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 7
M1 - 2195
ER -
ID: 35956515