TY - JOUR

T1 - Catalytic immunoglobulins as a marker of the humoral immune system pathology in schizophrenia

AU - Ermakov, E.

AU - Buneva, V.

AU - Smirnova, L.

AU - Ivanova, S.

AU - Nevinsky, Georgy A.

PY - 2020/11

Y1 - 2020/11

N2 - Background: Schizophrenia pathogenesis is thought to be associated with the immune system pathology and chronic low-grade inflammation [1]. Chronic inflammation can cause higher-order neural network disturbances, resulting in behavioural impairment [2]. The activation of inflammation is triggered by an increase in brain tissue of damage-associated molecular patterns (DAMPs) such as nucleic acids (DNA, RNA), histones and other molecules. One of the important signs of inflammation and impaired humoral immunity is the generation of catalytic antibodies (abzymes) that not only binding nucleic acids but also hydrolyze these molecules [3]. Catalytic antibodies were found among the total pool of immunoglobulins in autoimmune and viral diseases [3]. Recently, we discovered DNA-hydrolyzing antibodies in schizophrenia [4]. The level of catalytic activity of abzymes reflects the degree of pathology in the humoral immune system. In this work, we compared the level of catalytic activity of IgG antibodies in schizophrenia and in classic autoimmune diseases such as multiple sclerosis (MS) and systemic lupus erythematosus (SLE). Objective: To investigate and compare the level of catalytic activity of IgGs in the reaction of hydrolysis of nucleic acids (DNA and RNA) in schizophrenia, MS and SLE. Methods: In our study, 35 patients (17 men and 18 women) with a verified diagnosis of paranoid schizophrenia, 23 MS patients (5 men and 18 women), 12 SLE patients (6 men and 6 women) and 22 healthy donors (10 men and 12 women) were included. IgG preparations were obtained by affinity chromatography of the serum proteins on Protein-G-Sepharose. Catalytic activity of IgGs were revealed by the degree of hydrolysis of DNA, RNA and fluorescent labeled neuro-specific microRNAs (miR-137, miR-9, miR-219) as a substrate. The products of the hydrolysis were analyzed by electrophoresis using laser scanner. Statistical analysis was carried out using the Mann-Whitney U-test in the Statistica 10 software. Results: It was shown that the isolated pure IgG preparations possessed nuclease activity and hydrolyzed DNA and RNA in schizophrenia, MS, and SLE. DNA-hydrolyzing activity of healthy donors IgGs was 4-14 times lower (p

AB - Background: Schizophrenia pathogenesis is thought to be associated with the immune system pathology and chronic low-grade inflammation [1]. Chronic inflammation can cause higher-order neural network disturbances, resulting in behavioural impairment [2]. The activation of inflammation is triggered by an increase in brain tissue of damage-associated molecular patterns (DAMPs) such as nucleic acids (DNA, RNA), histones and other molecules. One of the important signs of inflammation and impaired humoral immunity is the generation of catalytic antibodies (abzymes) that not only binding nucleic acids but also hydrolyze these molecules [3]. Catalytic antibodies were found among the total pool of immunoglobulins in autoimmune and viral diseases [3]. Recently, we discovered DNA-hydrolyzing antibodies in schizophrenia [4]. The level of catalytic activity of abzymes reflects the degree of pathology in the humoral immune system. In this work, we compared the level of catalytic activity of IgG antibodies in schizophrenia and in classic autoimmune diseases such as multiple sclerosis (MS) and systemic lupus erythematosus (SLE). Objective: To investigate and compare the level of catalytic activity of IgGs in the reaction of hydrolysis of nucleic acids (DNA and RNA) in schizophrenia, MS and SLE. Methods: In our study, 35 patients (17 men and 18 women) with a verified diagnosis of paranoid schizophrenia, 23 MS patients (5 men and 18 women), 12 SLE patients (6 men and 6 women) and 22 healthy donors (10 men and 12 women) were included. IgG preparations were obtained by affinity chromatography of the serum proteins on Protein-G-Sepharose. Catalytic activity of IgGs were revealed by the degree of hydrolysis of DNA, RNA and fluorescent labeled neuro-specific microRNAs (miR-137, miR-9, miR-219) as a substrate. The products of the hydrolysis were analyzed by electrophoresis using laser scanner. Statistical analysis was carried out using the Mann-Whitney U-test in the Statistica 10 software. Results: It was shown that the isolated pure IgG preparations possessed nuclease activity and hydrolyzed DNA and RNA in schizophrenia, MS, and SLE. DNA-hydrolyzing activity of healthy donors IgGs was 4-14 times lower (p

UR - https://www.mendeley.com/catalogue/ca33af61-2bee-334f-ae30-3a211f86e33f/

U2 - 10.1016/j.euroneuro.2020.09.393

DO - 10.1016/j.euroneuro.2020.09.393

M3 - Meeting Abstract

VL - 40

SP - S303-S304

JO - European Neuropsychopharmacology

JF - European Neuropsychopharmacology

SN - 0924-977X

T2 - 33rd Congress of the European-College-of-Neuropsychopharmacology (ECNP)

Y2 - 12 September 2020 through 15 September 2020

ER -