Research output: Contribution to journal › Article › peer-review
Benign Pleural Mesothelial Cells Have Higher Osmotic Water Permeability than Malignant Pleural Mesothelioma Cells and Differentially Respond to Hyperosmolality. / Katkova, Liubov E.; Baturina, Galina S.; Bondar, Alexander A. et al.
In: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 52, No. 4, 01.01.2019, p. 869-878.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Benign Pleural Mesothelial Cells Have Higher Osmotic Water Permeability than Malignant Pleural Mesothelioma Cells and Differentially Respond to Hyperosmolality
AU - Katkova, Liubov E.
AU - Baturina, Galina S.
AU - Bondar, Alexander A.
AU - Jagirdar, Rajesh M.
AU - Hatzoglou, Chrissi
AU - Gourgoulianis, Konstantinos I.
AU - Solenov, Evgeniy I.
AU - Zarogiannis, Sotirios G.
N1 - Publisher Copyright: © 2019 Published The Author(s).
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Background/Aims: Cell volume regulation is a critical mechanism for cell homeostasis and depends on the osmotic water permeability (Pf) of the cell plasma membrane. The Pf of human mesothelial cells is unknown although they contribute to serosal fluid turnover. Methods: In this study we measured the osmotic water permeability of benign human mesothelial cells (MeT-5A) and of epithelioid (M14K) and sarcomatoid (ZL34) malignant pleural mesothelioma (MPM) cells in response to acute hyperosmotic stress. We also assessed the changes in their Pf after preconditioning with 4% glucose for 24 hours. In both cases we also assessed the role of AQP1 inhibition (0.1 mM HgCl2) on the Pf. Finally, we assessed corresponding changes in the AQP1 plasma membrane availability by immunofluorescence. Results: We report that MeT-5A cells have a significantly higher Pf as compared to M14K and ZL34 MPM cells [4.85E-03±2.37E-03 cm/sec (n=17) versus 2.74E-03±0.74E-03 cm/sec (n=11) and 2.86E-03±0.11E-03 cm/sec (n=11)]. AQP1 inhibition significantly decreased the Pf in all cells lines (p<0.001 in all cases). High glucose preconditioning for 24 hours significantly increased MeT-5A Pf (p<0.001), did not influence M14K Pf (p=0.19) and significantly reduced ZL34 Pf (p=0.02). Comparing cell lines after high glucose preconditioning, MeT-5A Pf was significantly higher than that of M14K and ZL34 MPM cells and the AQP1 inhibition effect was significant in MeT-5A and M14K cells. These results were corroborated by AQP1 immunofluorescence. Conclusion: We provide evidence for a differential regulation of Pf in benign and MPM cells that require further mechanistic investigation.
AB - Background/Aims: Cell volume regulation is a critical mechanism for cell homeostasis and depends on the osmotic water permeability (Pf) of the cell plasma membrane. The Pf of human mesothelial cells is unknown although they contribute to serosal fluid turnover. Methods: In this study we measured the osmotic water permeability of benign human mesothelial cells (MeT-5A) and of epithelioid (M14K) and sarcomatoid (ZL34) malignant pleural mesothelioma (MPM) cells in response to acute hyperosmotic stress. We also assessed the changes in their Pf after preconditioning with 4% glucose for 24 hours. In both cases we also assessed the role of AQP1 inhibition (0.1 mM HgCl2) on the Pf. Finally, we assessed corresponding changes in the AQP1 plasma membrane availability by immunofluorescence. Results: We report that MeT-5A cells have a significantly higher Pf as compared to M14K and ZL34 MPM cells [4.85E-03±2.37E-03 cm/sec (n=17) versus 2.74E-03±0.74E-03 cm/sec (n=11) and 2.86E-03±0.11E-03 cm/sec (n=11)]. AQP1 inhibition significantly decreased the Pf in all cells lines (p<0.001 in all cases). High glucose preconditioning for 24 hours significantly increased MeT-5A Pf (p<0.001), did not influence M14K Pf (p=0.19) and significantly reduced ZL34 Pf (p=0.02). Comparing cell lines after high glucose preconditioning, MeT-5A Pf was significantly higher than that of M14K and ZL34 MPM cells and the AQP1 inhibition effect was significant in MeT-5A and M14K cells. These results were corroborated by AQP1 immunofluorescence. Conclusion: We provide evidence for a differential regulation of Pf in benign and MPM cells that require further mechanistic investigation.
KW - AQP1
KW - Hyperosmotic stress
KW - Malignant pleural mesothelioma
KW - Osmotic water permeability
KW - Pleural mesothelial cells
KW - Aquaporin 1/metabolism
KW - Osmotic Pressure
KW - Humans
KW - Mesothelioma/metabolism
KW - Permeability
KW - Pleural Neoplasms/metabolism
KW - Cell Line, Tumor
KW - Pleura/metabolism
KW - Neoplasm Proteins/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85064503933&partnerID=8YFLogxK
U2 - 10.33594/000000060
DO - 10.33594/000000060
M3 - Article
C2 - 30958661
AN - SCOPUS:85064503933
VL - 52
SP - 869
EP - 878
JO - Cellular Physiology and Biochemistry
JF - Cellular Physiology and Biochemistry
SN - 1015-8987
IS - 4
ER -
ID: 19628622