Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish

Standard

Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish. / Wang, Dongmei; Yang, Long En; Wang, Jingtao et al.

In: Neurotoxicology and Teratology, Vol. 79, 106881, 01.05.2020.

Research output: Contribution to journal › Article › peer-review

Harvard

Wang, D, Yang, LE, Wang, J, Hu, G, Liu, ZY, Yan, D, Serikuly, N, Alpyshov, ET, Demin, KA, Galstyan, DS, Strekalova, T, de Abreu, MS, Amstislavskaya, TG & Kalueff, AV 2020, 'Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish', Neurotoxicology and Teratology, vol. 79, 106881. https://doi.org/10.1016/j.ntt.2020.106881

APA

Wang, D., Yang, L. E., Wang, J., Hu, G., Liu, Z. Y., Yan, D., Serikuly, N., Alpyshov, E. T., Demin, K. A., Galstyan, D. S., Strekalova, T., de Abreu, M. S., Amstislavskaya, T. G., & Kalueff, A. V. (2020). Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish. Neurotoxicology and Teratology, 79, [106881]. https://doi.org/10.1016/j.ntt.2020.106881

Vancouver

Wang D, Yang LE, Wang J, Hu G, Liu ZY, Yan D et al. Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish. Neurotoxicology and Teratology. 2020 May 1;79:106881. doi: 10.1016/j.ntt.2020.106881

Author

Wang, Dongmei ; Yang, Long En ; Wang, Jingtao et al. / Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish. In: Neurotoxicology and Teratology. 2020 ; Vol. 79.

BibTeX

@article{a5105019dc6145d99ab76db05e0ff3b2,

title = "Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish",

abstract = "Kava kava (Piper methysticum) is a medicinal plant containing kavalactones that exert potent sedative, analgesic and anti-stress action. However, their pharmacological effects and molecular targets remain poorly understood. The zebrafish (Danio rerio) has recently emerged as a powerful new model organism for neuroscience research and drug discovery. Here, we evaluate the effects of acute and chronic exposure to kava and kavalactones on adult zebrafish anxiety, aggression and sociality, as well as on their neurochemical, neuroendocrine and genomic responses. Supporting evolutionarily conserved molecular targets, acute kava and kavalactones evoked dose-dependent behavioral inhibition, upregulated brain expression of early protooncogenes c-fos and c-jun, elevated brain monoamines and lowered whole-body cortisol. Chronic 7-day kava exposure evoked similar behavioral effects, did not alter cortisol levels, and failed to evoke withdrawal-like states upon discontinuation. However, chronic kava upregulated several microglial (iNOS, Egr-2, CD11b), astrocytal (C3, C4B, S100a), epigenetic (ncoa-1) and pro-inflammatory (IL-1β, IL-6, TNFa) biomarker genes, downregulated CD206 and IL-4, and did not affect major apoptotic genes in the brain. Collectively, this study supports robust, evolutionarily conserved behavioral and physiological effects of kava and kavalactones in zebrafish, implicates brain monoamines in their acute effects, and provides novel important insights into potential role of neuroglial and epigenetic mechanisms in long-term kava use.",

keywords = "Behavior, Cortisol, Kava, Monoamines, Neuroglia, Zebrafish, SYMPTOMS, MODEL, RESPONSES, MICROGLIA, CORTEX, STRESS, WITHDRAWAL, EXTRACT",

author = "Dongmei Wang and Yang, {Long En} and Jingtao Wang and Guojun Hu and Liu, {Zi Yuan} and Dongni Yan and Nazar Serikuly and Alpyshov, {Erik T.} and Demin, {Konstantin A.} and Galstyan, {David S.} and Tatiana Strekalova and {de Abreu}, {Murilo S.} and Amstislavskaya, {Tamara G.} and Kalueff, {Allan V.}",

year = "2020",

month = may,

day = "1",

doi = "10.1016/j.ntt.2020.106881",

language = "English",

volume = "79",

journal = "Neurotoxicology and Teratology",

issn = "0892-0362",

publisher = "Elsevier Science Inc.",

}

RIS

TY - JOUR

T1 - Behavioral and physiological effects of acute and chronic kava exposure in adult zebrafish

AU - Wang, Dongmei

AU - Yang, Long En

AU - Wang, Jingtao

AU - Hu, Guojun

AU - Liu, Zi Yuan

AU - Yan, Dongni

AU - Serikuly, Nazar

AU - Alpyshov, Erik T.

AU - Demin, Konstantin A.

AU - Galstyan, David S.

AU - Strekalova, Tatiana

AU - de Abreu, Murilo S.

AU - Amstislavskaya, Tamara G.

AU - Kalueff, Allan V.

PY - 2020/5/1

Y1 - 2020/5/1

N2 - Kava kava (Piper methysticum) is a medicinal plant containing kavalactones that exert potent sedative, analgesic and anti-stress action. However, their pharmacological effects and molecular targets remain poorly understood. The zebrafish (Danio rerio) has recently emerged as a powerful new model organism for neuroscience research and drug discovery. Here, we evaluate the effects of acute and chronic exposure to kava and kavalactones on adult zebrafish anxiety, aggression and sociality, as well as on their neurochemical, neuroendocrine and genomic responses. Supporting evolutionarily conserved molecular targets, acute kava and kavalactones evoked dose-dependent behavioral inhibition, upregulated brain expression of early protooncogenes c-fos and c-jun, elevated brain monoamines and lowered whole-body cortisol. Chronic 7-day kava exposure evoked similar behavioral effects, did not alter cortisol levels, and failed to evoke withdrawal-like states upon discontinuation. However, chronic kava upregulated several microglial (iNOS, Egr-2, CD11b), astrocytal (C3, C4B, S100a), epigenetic (ncoa-1) and pro-inflammatory (IL-1β, IL-6, TNFa) biomarker genes, downregulated CD206 and IL-4, and did not affect major apoptotic genes in the brain. Collectively, this study supports robust, evolutionarily conserved behavioral and physiological effects of kava and kavalactones in zebrafish, implicates brain monoamines in their acute effects, and provides novel important insights into potential role of neuroglial and epigenetic mechanisms in long-term kava use.

AB - Kava kava (Piper methysticum) is a medicinal plant containing kavalactones that exert potent sedative, analgesic and anti-stress action. However, their pharmacological effects and molecular targets remain poorly understood. The zebrafish (Danio rerio) has recently emerged as a powerful new model organism for neuroscience research and drug discovery. Here, we evaluate the effects of acute and chronic exposure to kava and kavalactones on adult zebrafish anxiety, aggression and sociality, as well as on their neurochemical, neuroendocrine and genomic responses. Supporting evolutionarily conserved molecular targets, acute kava and kavalactones evoked dose-dependent behavioral inhibition, upregulated brain expression of early protooncogenes c-fos and c-jun, elevated brain monoamines and lowered whole-body cortisol. Chronic 7-day kava exposure evoked similar behavioral effects, did not alter cortisol levels, and failed to evoke withdrawal-like states upon discontinuation. However, chronic kava upregulated several microglial (iNOS, Egr-2, CD11b), astrocytal (C3, C4B, S100a), epigenetic (ncoa-1) and pro-inflammatory (IL-1β, IL-6, TNFa) biomarker genes, downregulated CD206 and IL-4, and did not affect major apoptotic genes in the brain. Collectively, this study supports robust, evolutionarily conserved behavioral and physiological effects of kava and kavalactones in zebrafish, implicates brain monoamines in their acute effects, and provides novel important insights into potential role of neuroglial and epigenetic mechanisms in long-term kava use.

KW - Behavior

KW - Cortisol

KW - Kava

KW - Monoamines

KW - Neuroglia

KW - Zebrafish

KW - SYMPTOMS

KW - MODEL

KW - RESPONSES

KW - MICROGLIA

KW - CORTEX

KW - STRESS

KW - WITHDRAWAL

KW - EXTRACT

UR - http://www.scopus.com/inward/record.url?scp=85084209463&partnerID=8YFLogxK

U2 - 10.1016/j.ntt.2020.106881

DO - 10.1016/j.ntt.2020.106881

M3 - Article

C2 - 32240749

AN - SCOPUS:85084209463

VL - 79

JO - Neurotoxicology and Teratology

JF - Neurotoxicology and Teratology

SN - 0892-0362

M1 - 106881

ER -

ID: 24230268