Research output: Contribution to journal › Article › peer-review
Bacterial Communities in Areas of Oil and Methane Seeps in Pelagic of Lake Baikal. / Zakharenko, Aleksandra S.; Galachyants, Yuriy P.; Morozov, Igor V. et al.
In: Microbial Ecology, Vol. 78, No. 2, 15.08.2019, p. 269-285.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Bacterial Communities in Areas of Oil and Methane Seeps in Pelagic of Lake Baikal
AU - Zakharenko, Aleksandra S.
AU - Galachyants, Yuriy P.
AU - Morozov, Igor V.
AU - Shubenkova, Olga V.
AU - Morozov, Alexey A.
AU - Ivanov, Vyacheslav G.
AU - Pimenov, Nikolay V.
AU - Krasnopeev, Andrey Y.
AU - Zemskaya, Tamara I.
N1 - Publisher Copyright: © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2019/8/15
Y1 - 2019/8/15
N2 - We have assessed the diversity of bacteria near oil-methane (area I) and methane (area II) seeps in the pelagic zone of Lake Baikal using massive parallel sequencing of 16S rRNA, pmoA, and mxaF gene fragments amplified from total DNA. At depths from the surface to 100 m, sequences belonging to Cyanobacteria dominated. In the communities to a depth of 200 m of the studied areas, Proteobacteria dominated the deeper layers of the water column. Alphaproteobacteria sequences were predominant in the community near the oil-methane seep, while the community near the methane seep was characterized by the prevalence of Alpha- and Gammaproteobacteria. Among representatives of these classes, type I methanotrophs prevailed in the 16S rRNA gene libraries from the near–bottom area, and type II methanotrophs were detected in minor quantities at different depths. In the analysis of the libraries of the pmoA and mxaF functional genes, we observed the different taxonomic composition of methanotrophic bacteria in the surface and deep layers of the water column. All pmoA sequences from area I were type II methanotrophs and were detected at a depth of 300 m, while sequences of type I methanotrophs were the most abundant in deep layers of the water column of area II. All mxaF gene sequences belonged to Methylobacterium representatives. Based on comparative analyses of 16S rRNA, pmoA, and mxaF gene fragment libraries, we suggest that there must be a wider spectrum of functional genes facilitating methane oxidation that were not detected with the primers used.
AB - We have assessed the diversity of bacteria near oil-methane (area I) and methane (area II) seeps in the pelagic zone of Lake Baikal using massive parallel sequencing of 16S rRNA, pmoA, and mxaF gene fragments amplified from total DNA. At depths from the surface to 100 m, sequences belonging to Cyanobacteria dominated. In the communities to a depth of 200 m of the studied areas, Proteobacteria dominated the deeper layers of the water column. Alphaproteobacteria sequences were predominant in the community near the oil-methane seep, while the community near the methane seep was characterized by the prevalence of Alpha- and Gammaproteobacteria. Among representatives of these classes, type I methanotrophs prevailed in the 16S rRNA gene libraries from the near–bottom area, and type II methanotrophs were detected in minor quantities at different depths. In the analysis of the libraries of the pmoA and mxaF functional genes, we observed the different taxonomic composition of methanotrophic bacteria in the surface and deep layers of the water column. All pmoA sequences from area I were type II methanotrophs and were detected at a depth of 300 m, while sequences of type I methanotrophs were the most abundant in deep layers of the water column of area II. All mxaF gene sequences belonged to Methylobacterium representatives. Based on comparative analyses of 16S rRNA, pmoA, and mxaF gene fragment libraries, we suggest that there must be a wider spectrum of functional genes facilitating methane oxidation that were not detected with the primers used.
KW - Diversity
KW - Lake Baikal
KW - Methanotrophic
KW - Methylotrophic bacteria
KW - Water column
KW - OXIDATION
KW - AUTOTROPHIC PICOPLANKTON
KW - ACIDOPHILIC BACTERIUM
KW - VERRUCOMICROBIA
KW - WATER COLUMN
KW - SP NOV.
KW - RICE FIELD
KW - LENA DELTA
KW - ABUNDANCE
KW - METHANOTROPHIC COMMUNITY
UR - http://www.scopus.com/inward/record.url?scp=85057315725&partnerID=8YFLogxK
U2 - 10.1007/s00248-018-1299-5
DO - 10.1007/s00248-018-1299-5
M3 - Article
C2 - 30483839
AN - SCOPUS:85057315725
VL - 78
SP - 269
EP - 285
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 2
ER -
ID: 17577672