Research output: Contribution to journal › Article › peer-review
Application of Parallel Reaction Monitoring to the Development and Validation of a Quantitative Assay for ST-246 in Human Plasma. / Chernonosov, Alexander A.; Oleinik, Galina A.; Koval, Vladimir V.
In: International Journal of Molecular Sciences, Vol. 23, No. 14, 8021, 21.07.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Application of Parallel Reaction Monitoring to the Development and Validation of a Quantitative Assay for ST-246 in Human Plasma
AU - Chernonosov, Alexander A.
AU - Oleinik, Galina A.
AU - Koval, Vladimir V.
N1 - Publisher Copyright: © 2022 by the authors.
PY - 2022/7/21
Y1 - 2022/7/21
N2 - In this work, we developed and validated a robust and sensitive method of liquid chromatography with high-resolution mass spectrometry in parallel reaction monitoring (PRM) mode for ST-246 (tecovirimat) quantification in human blood plasma. The method was compared with the multiple reaction monitoring (MRM) technique and showed better selectivity and similar sensitivity in a wider concentration range (10-5000 ng/mL). Within this range, intra- and interday variability of precision and accuracy were within acceptable ranges in accordance with the European Medicines Agency guidelines, and recovery was 87.9-100.6%. Samples were stable at 4 °C within 48 h and at -20 °C up to 3 months. The recovery and matrix effects in the proposed HRMS method were about 5% higher than those reported for the MRM method, but the PRM method showed better accuracy with comparable precision. It was found that the ST-246 concentration shown by the PRM method is approximately 24% higher than the output of the MRM one. Nonetheless, the high selectivity with similar sensitivity, as compared with traditional MRM methods, makes the proposed approach attractive for research and clinical use.
AB - In this work, we developed and validated a robust and sensitive method of liquid chromatography with high-resolution mass spectrometry in parallel reaction monitoring (PRM) mode for ST-246 (tecovirimat) quantification in human blood plasma. The method was compared with the multiple reaction monitoring (MRM) technique and showed better selectivity and similar sensitivity in a wider concentration range (10-5000 ng/mL). Within this range, intra- and interday variability of precision and accuracy were within acceptable ranges in accordance with the European Medicines Agency guidelines, and recovery was 87.9-100.6%. Samples were stable at 4 °C within 48 h and at -20 °C up to 3 months. The recovery and matrix effects in the proposed HRMS method were about 5% higher than those reported for the MRM method, but the PRM method showed better accuracy with comparable precision. It was found that the ST-246 concentration shown by the PRM method is approximately 24% higher than the output of the MRM one. Nonetheless, the high selectivity with similar sensitivity, as compared with traditional MRM methods, makes the proposed approach attractive for research and clinical use.
KW - blood plasma
KW - high-resolution mass spectrometry
KW - LC-HRMS
KW - PRM
KW - tecovirimat
UR - http://www.scopus.com/inward/record.url?scp=85135120524&partnerID=8YFLogxK
U2 - 10.3390/ijms23148021
DO - 10.3390/ijms23148021
M3 - Article
C2 - 35887368
AN - SCOPUS:85135120524
VL - 23
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 14
M1 - 8021
ER -
ID: 36744219