Research output: Contribution to journal › Article › peer-review
Appearances are deceptive : Three RNA viruses co-infected with the nucleopolyhedrovirus in host Lymantria dispar. / Pavlushin, Sergey V.; Ilinsky, Yury Yu; Belousova, Irina A. et al.
In: Virus Research, Vol. 297, 198371, 05.2021.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Appearances are deceptive
T2 - Three RNA viruses co-infected with the nucleopolyhedrovirus in host Lymantria dispar
AU - Pavlushin, Sergey V.
AU - Ilinsky, Yury Yu
AU - Belousova, Irina A.
AU - Bayborodin, Sergey I.
AU - Lunev, Evgenii A.
AU - Kechin, Andrey A.
AU - Khrapov, Evgeniy A.
AU - Filipenko, Maksim L.
AU - Toshchakov, Stepan V.
AU - Martemyanov, Vyacheslav V.
N1 - Publisher Copyright: © 2021 Elsevier B.V.
PY - 2021/5
Y1 - 2021/5
N2 - The virus infection, which visually looks like typical monoinfection, in fact may hide a great complex of different species. Without detailed analysis, we may miss the important interaction between pathogens, including new species. In the current study, we found the new species inside the mix of cubic and polyhedral occlusion bodies (OBs) isolated from the gypsy moth, Lymantria dispar L. (Ld). Transmission electron microscopy (TEM) revealed that into the one cadaver were OBs which belonged to baculovirus and cypoviruses. The baculovirus produced polyhedral OBs, while cypoviruses produced polyhedral and cubic OBs. Genomic analysis detected the multiple Ld nucleopolyhedroviruses, and cypoviruses were Hubei lepidoptera virus 3 and Dendrolimus punctatus cypovirus 1. This represents the first isolation of the Hubei lepidoptera virus 3 from the gypsy moth, proposed as “Lymantria dispar cypovirus 3”. The RNAseq analysis also revealed the presence of Lymantria dispar iflavirus 1. The insecticidal activity of the mixed infection was comparable to that of typical baculovirus monoinfection. Thus, we demonstrate that i) the shape of OBs identified by light microscopy cannot be a robust indicator of viral species infecting the host; ii) only specific analysis may reveal the true composition of viral infection.
AB - The virus infection, which visually looks like typical monoinfection, in fact may hide a great complex of different species. Without detailed analysis, we may miss the important interaction between pathogens, including new species. In the current study, we found the new species inside the mix of cubic and polyhedral occlusion bodies (OBs) isolated from the gypsy moth, Lymantria dispar L. (Ld). Transmission electron microscopy (TEM) revealed that into the one cadaver were OBs which belonged to baculovirus and cypoviruses. The baculovirus produced polyhedral OBs, while cypoviruses produced polyhedral and cubic OBs. Genomic analysis detected the multiple Ld nucleopolyhedroviruses, and cypoviruses were Hubei lepidoptera virus 3 and Dendrolimus punctatus cypovirus 1. This represents the first isolation of the Hubei lepidoptera virus 3 from the gypsy moth, proposed as “Lymantria dispar cypovirus 3”. The RNAseq analysis also revealed the presence of Lymantria dispar iflavirus 1. The insecticidal activity of the mixed infection was comparable to that of typical baculovirus monoinfection. Thus, we demonstrate that i) the shape of OBs identified by light microscopy cannot be a robust indicator of viral species infecting the host; ii) only specific analysis may reveal the true composition of viral infection.
KW - Baculovirus
KW - Genome sequence
KW - Hubei lepidoptera virus 3
KW - Iflaviridae
KW - Mix infection
KW - Reoviridae
UR - http://www.scopus.com/inward/record.url?scp=85102355651&partnerID=8YFLogxK
U2 - 10.1016/j.virusres.2021.198371
DO - 10.1016/j.virusres.2021.198371
M3 - Article
C2 - 33684420
AN - SCOPUS:85102355651
VL - 297
JO - Virus Research
JF - Virus Research
SN - 0168-1702
M1 - 198371
ER -
ID: 28079855