TY - JOUR

T1 - Anion influence on the structure and cytotoxicity of Copper(II) complexes based on 2,2’-bipyridine/1,10-phenanthroline and benzimidazole derivative

AU - Golubeva, Julia A.

AU - Ермакова, Екатерина Андреевна

AU - Smirnova, K. S.

AU - Klyushova, Lyubov S.

AU - Зайцев, Никита Павлович

AU - Осик, Наталья Алексеевна

AU - Berezin, Alexey S.

AU - Потапов, Андрей Сергеевич

AU - Лидер, Елизавета Викторовна

N1 - The research (Nikolaev Institute of Inorganic Chemistry SB RAS) was supported by the Ministry of Science and Higher Education of the Russian Federation, No. 121031700321-3. The investigation of biological activity was performed on the equipment of the Center for Collective Use “Proteomic Analysis” (FRC FTM) and supported by the Ministry of Science and Higher Education of the Russian Federation, No. 125031203556-7. The authors thank the Ministry of Science and Higher Education of the Russian Federation for the access to the MS equipment. (project #AAAA-A21-121012290043-3).

PY - 2025/11/1

Y1 - 2025/11/1

N2 - Eight new copper(II) coordination compounds have been synthesized by the reactions of Cu(X)2 salts (X = Cl−, Br−, NO3− or ClO4−), 2,2′-bipyridine / 1,10-phenanthroline and 1-(1H-benzimidazol-1-ylmethyl)-1H-1,2,3-benzotriazole (L): [Cu(bipy/phen)LCl2]·H2O (1)/(5), [Cu(bipy/phen)LBr2]·H2O (2)/(6), Cu(bipy)L2(NO3)2(H2O) (3), [Cu(bipy)L2(ClO4)2]·0.4C2H5OH (4), {[Cu(phen)L2(NO3)]NO3·0.5H2O·0.8C2H5OH}n (7), [Cu(phen)L2(MeCN)ClO4]ClO4 (8). The complexes were characterized by CHN, thermogravimetric and high-resolution electrospray mass spectrometric analysis, IR spectroscopy, powder and single-crystal X-ray diffraction. A crystallographic study revealed that Cu:bipy/phen:L ratio, the geometry of the complexes and their nuclearity differ depending on the selected anions. Stability of the complexes in ethanol and phosphate-buffered saline was studied by UV–Vis and EPR spectroscopy. In vitro cytotoxic activity was determined against three tumor cell lines (A549, Hep2, HepG2) and non-tumor lung fibroblasts MRC5. All complexes exhibit dose-dependent cytotoxicity on tumor cells with phenanthroline-based compounds 5–8 being 3–10 times more cytotoxic than medical drug cisplatin.

AB - Eight new copper(II) coordination compounds have been synthesized by the reactions of Cu(X)2 salts (X = Cl−, Br−, NO3− or ClO4−), 2,2′-bipyridine / 1,10-phenanthroline and 1-(1H-benzimidazol-1-ylmethyl)-1H-1,2,3-benzotriazole (L): [Cu(bipy/phen)LCl2]·H2O (1)/(5), [Cu(bipy/phen)LBr2]·H2O (2)/(6), Cu(bipy)L2(NO3)2(H2O) (3), [Cu(bipy)L2(ClO4)2]·0.4C2H5OH (4), {[Cu(phen)L2(NO3)]NO3·0.5H2O·0.8C2H5OH}n (7), [Cu(phen)L2(MeCN)ClO4]ClO4 (8). The complexes were characterized by CHN, thermogravimetric and high-resolution electrospray mass spectrometric analysis, IR spectroscopy, powder and single-crystal X-ray diffraction. A crystallographic study revealed that Cu:bipy/phen:L ratio, the geometry of the complexes and their nuclearity differ depending on the selected anions. Stability of the complexes in ethanol and phosphate-buffered saline was studied by UV–Vis and EPR spectroscopy. In vitro cytotoxic activity was determined against three tumor cell lines (A549, Hep2, HepG2) and non-tumor lung fibroblasts MRC5. All complexes exhibit dose-dependent cytotoxicity on tumor cells with phenanthroline-based compounds 5–8 being 3–10 times more cytotoxic than medical drug cisplatin.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-105007516739&origin=inward&txGid=2cf59a8cfa16525276185df65b7d8918

U2 - 10.1016/j.ica.2025.122803

DO - 10.1016/j.ica.2025.122803

M3 - Article

VL - 587

JO - Inorganica Chimica Acta

JF - Inorganica Chimica Acta

SN - 0020-1693

M1 - 122803

ER -