Standard

Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis. / Fokina, Alesya; Wang, Meiling; Ilyina, Anna et al.

In: Analytical Biochemistry, Vol. 555, 15.08.2018, p. 9-11.

Research output: Contribution to journal › Article › peer-review

Harvard

Fokina, A, Wang, M, Ilyina, A, Klabenkova, K, Burakova, E, Chelobanov, B & Stetsenko, D 2018, 'Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis', Analytical Biochemistry, vol. 555, pp. 9-11. https://doi.org/10.1016/j.ab.2018.05.027

APA

Fokina, A., Wang, M., Ilyina, A., Klabenkova, K., Burakova, E., Chelobanov, B., & Stetsenko, D. (2018). Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis. Analytical Biochemistry, 555, 9-11. https://doi.org/10.1016/j.ab.2018.05.027

Vancouver

Fokina A, Wang M, Ilyina A, Klabenkova K, Burakova E, Chelobanov B et al. Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis. Analytical Biochemistry. 2018 Aug 15;555:9-11. doi: 10.1016/j.ab.2018.05.027

Author

Fokina, Alesya ; Wang, Meiling ; Ilyina, Anna et al. / Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis. In: Analytical Biochemistry. 2018 ; Vol. 555. pp. 9-11.

BibTeX

@article{77d87b106ce14ce48701552e8c33d5ff,
title = "Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis",
abstract = "Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.",
keywords = "DNA/analysis, Electrophoresis, Polyacrylamide Gel, Guanidine/chemistry, Oligonucleotides/analysis, RNA/analysis",
author = "Alesya Fokina and Meiling Wang and Anna Ilyina and Kristina Klabenkova and Ekaterina Burakova and Boris Chelobanov and Dmitry Stetsenko",
note = "Copyright {\textcopyright} 2018. Published by Elsevier Inc.",
year = "2018",
month = aug,
day = "15",
doi = "10.1016/j.ab.2018.05.027",
language = "English",
volume = "555",
pages = "9--11",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Analysis of new charge-neutral DNA/RNA analogues phosphoryl guanidine oligonucleotides (PGO) by gel electrophoresis

AU - Fokina, Alesya

AU - Wang, Meiling

AU - Ilyina, Anna

AU - Klabenkova, Kristina

AU - Burakova, Ekaterina

AU - Chelobanov, Boris

AU - Stetsenko, Dmitry

N1 - Copyright © 2018. Published by Elsevier Inc.

PY - 2018/8/15

Y1 - 2018/8/15

N2 - Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.

AB - Analysis and isolation of new charge-neutral phosphoryl guanidine oligonucleotides (PGO) by vertical slab electrophoresis were tested at different pH values (3–11) or in the presence of SDS as a micelle-forming agent. The most convenient way to analyze and purify phosphoryl guanidine oligonucleotides was by denaturing PAGE (8 M urea) at pH 3. The mobility of PGO is dependent on their A + C content. To analyze PGO containing only G, T or U, denaturing PAGE at pH 11 can be used, although the conditions need to be optimized. Bands were visualized by UV shadowing or Coomassie Brilliant Blue staining.

KW - DNA/analysis

KW - Electrophoresis, Polyacrylamide Gel

KW - Guanidine/chemistry

KW - Oligonucleotides/analysis

KW - RNA/analysis

UR - http://www.scopus.com/inward/record.url?scp=85048163507&partnerID=8YFLogxK

U2 - 10.1016/j.ab.2018.05.027

DO - 10.1016/j.ab.2018.05.027

M3 - Article

C2 - 29864402

AN - SCOPUS:85048163507

VL - 555

SP - 9

EP - 11

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

ER -

ID: 13794884