Research output: Contribution to journal › Article › peer-review
A TaqMan Assay for the Detection and Monitoring of Potentially Invasive Lasiocampids, With Particular Attention to the Siberian Silk Moth, Dendrolimus sibiricus (Lepidoptera: Lasiocampidae). / Stewart, Don; Djoumad, Abdelmadjid; Holden, Dave et al.
In: Journal of insect science, Vol. 23, No. 1, 5, 01.01.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A TaqMan Assay for the Detection and Monitoring of Potentially Invasive Lasiocampids, With Particular Attention to the Siberian Silk Moth, Dendrolimus sibiricus (Lepidoptera: Lasiocampidae)
AU - Stewart, Don
AU - Djoumad, Abdelmadjid
AU - Holden, Dave
AU - Kimoto, Troy
AU - Capron, Arnaud
AU - Dubatolov, Vladimir V.
AU - Akhanaev, Yuriy B.
AU - Yakimova, Maria E.
AU - Martemyanov, Vyacheslav V.
AU - Cusson, Michel
N1 - We are grateful to A. Naaum (NatureMetrics, Guelph, Canada) for insightful suggestions regarding the development of the on-site version of our assay. We thank colleagues who provided insect specimens for this study; their names are listed in ]. This work was supported by a grant from the Genomics Research and Development Initiative (Government of Canada) to MC and by grants from the Russian Science Foundation to V. V. M. and V. V. D. The work reported here was completed in 2021.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - The Siberian silk moth, Dendrolimus sibiricus Tschetverikov, is a very serious pest of conifers in Russia and is an emerging threat in North America where an accidental introduction could have devastating impacts on native forest resources. Other Dendrolimus Germar species and related Eurasian lasiocampids in the genus Malacosoma (Hubner) could also present a risk to North America's forests. Foreign vessels entering Canadian and U.S. ports are regularly inspected for Lymantria dispar (Linnaeus) and for the presence of other potentially invasive insects, including suspicious lasiocampid eggs. However, eggs are difficult to identify based on morphological features alone. Here, we report on the development of two TaqMan (Roche Molecular Systems, Inc., Rotkreuz, Switzerland) assays designed to assist regulatory agencies in their identification of these insects. Developed using the barcode region of the cytochrome c oxidase I (COI) gene and run in triplex format, the first assay can detect Dendrolimus and Malacosoma DNA, and can distinguish North American from Eurasian Malacosoma species. The second assay is based on markers identified within the internal transcribed spacer 2 (ITS2) region and was designed to specifically identify D. sibiricus, while discriminating closely related Dendrolimus taxa. In addition to providing direct species identification in the context of its use in North America, the D. sibiricus assay should prove useful for monitoring the spread of this pest in Eurasia, where its range overlaps with those of the morphologically identical D. superans (Butler) and similar D. pini (Linnaeus). The assays described here can be performed either in the lab on a benchtop instrument, or on-site using a portable machine.
AB - The Siberian silk moth, Dendrolimus sibiricus Tschetverikov, is a very serious pest of conifers in Russia and is an emerging threat in North America where an accidental introduction could have devastating impacts on native forest resources. Other Dendrolimus Germar species and related Eurasian lasiocampids in the genus Malacosoma (Hubner) could also present a risk to North America's forests. Foreign vessels entering Canadian and U.S. ports are regularly inspected for Lymantria dispar (Linnaeus) and for the presence of other potentially invasive insects, including suspicious lasiocampid eggs. However, eggs are difficult to identify based on morphological features alone. Here, we report on the development of two TaqMan (Roche Molecular Systems, Inc., Rotkreuz, Switzerland) assays designed to assist regulatory agencies in their identification of these insects. Developed using the barcode region of the cytochrome c oxidase I (COI) gene and run in triplex format, the first assay can detect Dendrolimus and Malacosoma DNA, and can distinguish North American from Eurasian Malacosoma species. The second assay is based on markers identified within the internal transcribed spacer 2 (ITS2) region and was designed to specifically identify D. sibiricus, while discriminating closely related Dendrolimus taxa. In addition to providing direct species identification in the context of its use in North America, the D. sibiricus assay should prove useful for monitoring the spread of this pest in Eurasia, where its range overlaps with those of the morphologically identical D. superans (Butler) and similar D. pini (Linnaeus). The assays described here can be performed either in the lab on a benchtop instrument, or on-site using a portable machine.
KW - Dendrolimus
KW - Lasiocampidae
KW - Malacosoma
KW - TaqMan
KW - molecular identification
KW - Canada
KW - Animals
KW - Insecta
KW - Moths/genetics
KW - Ovum
KW - Manduca
KW - Bombyx
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85147318047&origin=inward&txGid=cf1512d94717988595c0800e29f2d383
UR - https://www.mendeley.com/catalogue/f573ca67-f0d7-35f4-adf9-6c7381b571d5/
U2 - 10.1093/jisesa/ieac062
DO - 10.1093/jisesa/ieac062
M3 - Article
C2 - 36723233
VL - 23
JO - Journal of insect science
JF - Journal of insect science
SN - 1536-2442
IS - 1
M1 - 5
ER -
ID: 59340167