Research output: Contribution to journal › Article › peer-review
A Spontaneous Inversion of the X Chromosome Heterochromatin Provides a Tool for Studying the Structure and Activity of the Nucleolus in Drosophila melanogaster. / Kolesnikova, Tatyana D.; Klenov, Mikhail S.; Nokhova, Alina R. et al.
In: Cells, Vol. 11, No. 23, 3872, 12.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A Spontaneous Inversion of the X Chromosome Heterochromatin Provides a Tool for Studying the Structure and Activity of the Nucleolus in Drosophila melanogaster
AU - Kolesnikova, Tatyana D.
AU - Klenov, Mikhail S.
AU - Nokhova, Alina R.
AU - Lavrov, Sergey A.
AU - Pokholkova, Galina V.
AU - Schubert, Veit
AU - Maltseva, Svetlana V.
AU - Cook, Kevin R.
AU - Dixon, Michael J.
AU - Zhimulev, Igor F.
N1 - Funding Information: The part of the work associated with nanopore sequencing was funded by the Russian Science Foundation (RSF) (grant number 19-74-20178). Part of the work related to the visualization of nucleolar components was supported by a grant from the Russian Science Foundation (19-14-00382). The part of the work associated with ultrastructural polytene chromosome analysis was funded by a joint Russian-German grant from the Russian Foundation for Basic Research (No. 20-54-12016) and the Deutsche Forschungsgemeinschaft (Schu762/12-1). The part of the work associated with the new inversion cytological mapping was funded by the Russian Science Foundation grant No. 19-14-00051. Work at the Bloomington Drosophila Stock Center was supported by the U.S. National Institutes of Health grant P40 OD018537. Publisher Copyright: © 2022 by the authors.
PY - 2022/12
Y1 - 2022/12
N2 - The pericentromeric heterochromatin is largely composed of repetitive sequences, making it difficult to analyze with standard molecular biological methods. At the same time, it carries many functional elements with poorly understood mechanisms of action. The search for new experimental models for the analysis of heterochromatin is an urgent task. In this work, we used the Rif1 mutation, which suppresses the underreplication of all types of repeated sequences, to analyze heterochromatin regions in polytene chromosomes of Drosophila melanogaster. In the Rif1 background, we discovered and described in detail a new inversion, In(1)19EHet, which arose on a chromosome already carrying the In(1)sc8 inversion and transferred a large part of X chromosome heterochromatin, including the nucleolar organizer to a new euchromatic environment. Using nanopore sequencing and FISH, we have identified the eu- and heterochromatin breakpoints of In(1)19EHet. The combination of the new inversion and the Rif1 mutation provides a promising tool for studies of X chromosome heterochromatin structure, nucleolar organization, and the nucleolar dominance phenomenon. In particular, we found that, with the complete polytenization of rDNA repeats, the nucleolus consists of a cloud-like structure corresponding to the classical nucleolus of polytene chromosomes, as well as an unusual intrachromosomal structure containing alternating transcriptionally active and inactive regions.
AB - The pericentromeric heterochromatin is largely composed of repetitive sequences, making it difficult to analyze with standard molecular biological methods. At the same time, it carries many functional elements with poorly understood mechanisms of action. The search for new experimental models for the analysis of heterochromatin is an urgent task. In this work, we used the Rif1 mutation, which suppresses the underreplication of all types of repeated sequences, to analyze heterochromatin regions in polytene chromosomes of Drosophila melanogaster. In the Rif1 background, we discovered and described in detail a new inversion, In(1)19EHet, which arose on a chromosome already carrying the In(1)sc8 inversion and transferred a large part of X chromosome heterochromatin, including the nucleolar organizer to a new euchromatic environment. Using nanopore sequencing and FISH, we have identified the eu- and heterochromatin breakpoints of In(1)19EHet. The combination of the new inversion and the Rif1 mutation provides a promising tool for studies of X chromosome heterochromatin structure, nucleolar organization, and the nucleolar dominance phenomenon. In particular, we found that, with the complete polytenization of rDNA repeats, the nucleolus consists of a cloud-like structure corresponding to the classical nucleolus of polytene chromosomes, as well as an unusual intrachromosomal structure containing alternating transcriptionally active and inactive regions.
KW - Drosophila melanogaster
KW - heterochromatin
KW - In(1)sc
KW - inversion
KW - nucleolus
KW - polytene chromosomes
KW - Rif1
KW - underreplication
KW - Heterochromatin/genetics
KW - Drosophila Proteins/genetics
KW - Drosophila melanogaster/genetics
KW - Repetitive Sequences, Nucleic Acid/genetics
KW - X Chromosome/genetics
KW - Animals
KW - Nucleolus Organizer Region
KW - Carrier Proteins/genetics
KW - In(1)sc8
UR - http://www.scopus.com/inward/record.url?scp=85143657245&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/43bbe341-6604-350f-9232-b3badbc971a9/
U2 - 10.3390/cells11233872
DO - 10.3390/cells11233872
M3 - Article
C2 - 36497131
AN - SCOPUS:85143657245
VL - 11
JO - Cells
JF - Cells
SN - 2073-4409
IS - 23
M1 - 3872
ER -
ID: 40848111