Standard

A novel method of sample homogenization with the use of a microtome-cryostat apparatus. / Zelentsova, Ekaterina A.; Yanshole, Vadim V.; Tsentalovich, Yuri P.

In: RSC Advances, Vol. 9, No. 65, 21.11.2019, p. 37809-37817.

Research output: Contribution to journal › Article › peer-review

Harvard

Zelentsova, EA, Yanshole, VV & Tsentalovich, YP 2019, 'A novel method of sample homogenization with the use of a microtome-cryostat apparatus', RSC Advances, vol. 9, no. 65, pp. 37809-37817. https://doi.org/10.1039/c9ra06808b

APA

Zelentsova, E. A., Yanshole, V. V., & Tsentalovich, Y. P. (2019). A novel method of sample homogenization with the use of a microtome-cryostat apparatus. RSC Advances, 9(65), 37809-37817. https://doi.org/10.1039/c9ra06808b

Vancouver

Zelentsova EA, Yanshole VV, Tsentalovich YP. A novel method of sample homogenization with the use of a microtome-cryostat apparatus. RSC Advances. 2019 Nov 21;9(65):37809-37817. doi: 10.1039/c9ra06808b

Author

Zelentsova, Ekaterina A. ; Yanshole, Vadim V. ; Tsentalovich, Yuri P. / A novel method of sample homogenization with the use of a microtome-cryostat apparatus. In: RSC Advances. 2019 ; Vol. 9, No. 65. pp. 37809-37817.

BibTeX

@article{220c2c6d490d4eb1a437087d5b18a4e4,
title = "A novel method of sample homogenization with the use of a microtome-cryostat apparatus",
abstract = "Quantitative metabolomics places high demands on sample preparation, including a high degree of metabolite extraction and controlled sample weight. In respect to elastic collagen-rich tissues, the existing methods of sample homogenization poorly fit these demands due to incomplete homogenization, sample material loss, or metabolite degradation. Herein, a novel method based on the use of a microtome-cryostat apparatus is proposed. The performance of the cryotome method is compared with the results obtained with the use of a vortex bead beating. NMR-based metabolomic analysis shows that the extraction efficiency and the data scattering for both methods of sample preparation are similar. However, the heat generation during the bead beating causes the destruction of thermally-unstable compounds; besides, it may cause protein hydrolysis, leading to an artificial increase in the amino acid level. The cryotome method of sample homogenization does not cause sample heating, and it seems to be ideal for elastic tissues.",
keywords = "NUCLEAR-MAGNETIC-RESONANCE, EXTRACTION STRATEGIES, METABOLITE EXTRACTION, METABOLOMICS, NMR, SERUM, QUANTIFICATION, DEGRADATION, COENZYMES",
author = "Zelentsova, {Ekaterina A.} and Yanshole, {Vadim V.} and Tsentalovich, {Yuri P.}",
note = "This journal is {\textcopyright} The Royal Society of Chemistry.",
year = "2019",
month = nov,
day = "21",
doi = "10.1039/c9ra06808b",
language = "English",
volume = "9",
pages = "37809--37817",
journal = "RSC Advances",
issn = "2046-2069",
publisher = "ROYAL SOC CHEMISTRY",
number = "65",

}

RIS

TY - JOUR

T1 - A novel method of sample homogenization with the use of a microtome-cryostat apparatus

AU - Zelentsova, Ekaterina A.

AU - Yanshole, Vadim V.

AU - Tsentalovich, Yuri P.

N1 - This journal is © The Royal Society of Chemistry.

PY - 2019/11/21

Y1 - 2019/11/21

N2 - Quantitative metabolomics places high demands on sample preparation, including a high degree of metabolite extraction and controlled sample weight. In respect to elastic collagen-rich tissues, the existing methods of sample homogenization poorly fit these demands due to incomplete homogenization, sample material loss, or metabolite degradation. Herein, a novel method based on the use of a microtome-cryostat apparatus is proposed. The performance of the cryotome method is compared with the results obtained with the use of a vortex bead beating. NMR-based metabolomic analysis shows that the extraction efficiency and the data scattering for both methods of sample preparation are similar. However, the heat generation during the bead beating causes the destruction of thermally-unstable compounds; besides, it may cause protein hydrolysis, leading to an artificial increase in the amino acid level. The cryotome method of sample homogenization does not cause sample heating, and it seems to be ideal for elastic tissues.

AB - Quantitative metabolomics places high demands on sample preparation, including a high degree of metabolite extraction and controlled sample weight. In respect to elastic collagen-rich tissues, the existing methods of sample homogenization poorly fit these demands due to incomplete homogenization, sample material loss, or metabolite degradation. Herein, a novel method based on the use of a microtome-cryostat apparatus is proposed. The performance of the cryotome method is compared with the results obtained with the use of a vortex bead beating. NMR-based metabolomic analysis shows that the extraction efficiency and the data scattering for both methods of sample preparation are similar. However, the heat generation during the bead beating causes the destruction of thermally-unstable compounds; besides, it may cause protein hydrolysis, leading to an artificial increase in the amino acid level. The cryotome method of sample homogenization does not cause sample heating, and it seems to be ideal for elastic tissues.

KW - NUCLEAR-MAGNETIC-RESONANCE

KW - EXTRACTION STRATEGIES

KW - METABOLITE EXTRACTION

KW - METABOLOMICS

KW - NMR

KW - SERUM

KW - QUANTIFICATION

KW - DEGRADATION

KW - COENZYMES

UR - http://www.scopus.com/inward/record.url?scp=85075724690&partnerID=8YFLogxK

U2 - 10.1039/c9ra06808b

DO - 10.1039/c9ra06808b

M3 - Article

C2 - 35541765

AN - SCOPUS:85075724690

VL - 9

SP - 37809

EP - 37817

JO - RSC Advances

JF - RSC Advances

SN - 2046-2069

IS - 65

ER -

ID: 22499060