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A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets. / Tikhonova, Maria A.; Maslov, Nikolai A.; Bashirzade, Alim A. et al.

In: Pharmaceutics, Vol. 14, No. 8, 1751, 22.08.2022.

Research output: Contribution to journal › Article › peer-review

Harvard

Tikhonova, MA, Maslov, NA, Bashirzade, AA, Nehoroshev, EV, Babchenko, VY, Chizhova, ND, Tsibulskaya, EO, Akopyan, AA, Markova, EV, Yang, YL, Lu, KT, Kalueff, AV, Aftanas, LI & Amstislavskaya, TG 2022, 'A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets', Pharmaceutics, vol. 14, no. 8, 1751. https://doi.org/10.3390/pharmaceutics14081751

APA

Tikhonova, M. A., Maslov, N. A., Bashirzade, A. A., Nehoroshev, E. V., Babchenko, V. Y., Chizhova, N. D., Tsibulskaya, E. O., Akopyan, A. A., Markova, E. V., Yang, Y. L., Lu, K. T., Kalueff, A. V., Aftanas, L. I., & Amstislavskaya, T. G. (2022). A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets. Pharmaceutics, 14(8), [1751]. https://doi.org/10.3390/pharmaceutics14081751

Vancouver

Tikhonova MA, Maslov NA, Bashirzade AA, Nehoroshev EV, Babchenko VY, Chizhova ND et al. A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets. Pharmaceutics. 2022 Aug 22;14(8):1751. doi: 10.3390/pharmaceutics14081751

Author

Tikhonova, Maria A. ; Maslov, Nikolai A. ; Bashirzade, Alim A. et al. / A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets. In: Pharmaceutics. 2022 ; Vol. 14, No. 8.

BibTeX

@article{2f42194b027640e4a5a7482afdc257d8,
title = "A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets",
abstract = "Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening.",
keywords = "animal model, behavior, laser, neurodegeneration, neuroinflammation, neurorepair, traumatic brain injury, zebrafish",
author = "Tikhonova, {Maria A.} and Maslov, {Nikolai A.} and Bashirzade, {Alim A.} and Nehoroshev, {Eugenyi V.} and Babchenko, {Vladislav Y.} and Chizhova, {Nadezhda D.} and Tsibulskaya, {Elena O.} and Akopyan, {Anna A.} and Markova, {Evgeniya V.} and Yang, {Yi Ling} and Lu, {Kwok Tung} and Kalueff, {Allan V.} and Aftanas, {Lyubomir I.} and Amstislavskaya, {Tamara G.}",
note = "Funding Information: We thank Alisa S. Belova for technical support in experimental manipulations and cortisol assay. We also thank Anatoly A. Maslov for the idea of using laser radiation to introduce brain damage. A.V.K. lab is supported by St. Peterburg State University funds (Pure ID 73026081). Funding Information: This study was funded by Russian Science Foundation (grant No. 20-65-46006). Publisher Copyright: {\textcopyright} 2022 by the authors.",
year = "2022",
month = aug,
day = "22",
doi = "10.3390/pharmaceutics14081751",
language = "English",
volume = "14",
journal = "Pharmaceutics",
issn = "1999-4923",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "8",

}

RIS

TY - JOUR

T1 - A Novel Laser-Based Zebrafish Model for Studying Traumatic Brain Injury and Its Molecular Targets

AU - Tikhonova, Maria A.

AU - Maslov, Nikolai A.

AU - Bashirzade, Alim A.

AU - Nehoroshev, Eugenyi V.

AU - Babchenko, Vladislav Y.

AU - Chizhova, Nadezhda D.

AU - Tsibulskaya, Elena O.

AU - Akopyan, Anna A.

AU - Markova, Evgeniya V.

AU - Yang, Yi Ling

AU - Lu, Kwok Tung

AU - Kalueff, Allan V.

AU - Aftanas, Lyubomir I.

AU - Amstislavskaya, Tamara G.

N1 - Funding Information: We thank Alisa S. Belova for technical support in experimental manipulations and cortisol assay. We also thank Anatoly A. Maslov for the idea of using laser radiation to introduce brain damage. A.V.K. lab is supported by St. Peterburg State University funds (Pure ID 73026081). Funding Information: This study was funded by Russian Science Foundation (grant No. 20-65-46006). Publisher Copyright: © 2022 by the authors.

PY - 2022/8/22

Y1 - 2022/8/22

N2 - Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening.

AB - Traumatic brain injury (TBI) is a major public health problem. Here, we developed a novel model of non-invasive TBI induced by laser irradiation in the telencephalon of adult zebrafish (Danio rerio) and assessed their behavior and neuromorphology to validate the model and evaluate potential targets for neuroreparative treatment. Overall, TBI induced hypolocomotion and anxiety-like behavior in the novel tank test, strikingly recapitulating responses in mammalian TBI models, hence supporting the face validity of our model. NeuN-positive cell staining was markedly reduced one day, but not seven days, after TBI, suggesting increased neuronal damage immediately after the injury, and its fast recovery. The brain-derived neurotrophic factor (Bdnf) level in the brain dropped immediately after the trauma, but fully recovered seven days later. A marker of microglial activation, Iba1, was elevated in the TBI brain, albeit decreasing from Day 3. The levels of hypoxia-inducible factor 1-alpha (Hif1a) increased 30 min after the injury, and recovered by Day 7, further supporting the construct validity of the model. Collectively, these findings suggest that our model of laser-induced brain injury in zebrafish reproduces mild TBI and can be a useful tool for TBI research and preclinical neuroprotective drug screening.

KW - animal model

KW - behavior

KW - laser

KW - neurodegeneration

KW - neuroinflammation

KW - neurorepair

KW - traumatic brain injury

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85137388324&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/b1389498-88ec-371f-905e-544f9c8b3fa6/

U2 - 10.3390/pharmaceutics14081751

DO - 10.3390/pharmaceutics14081751

M3 - Article

C2 - 36015377

AN - SCOPUS:85137388324

VL - 14

JO - Pharmaceutics

JF - Pharmaceutics

SN - 1999-4923

IS - 8

M1 - 1751

ER -

ID: 37123990