A new enterobacter cloacae bacteriophage ec151 encodes the deazaguanine dna modification pathway and represents a new genus within the siphoviridae family

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A new enterobacter cloacae bacteriophage ec151 encodes the deazaguanine dna modification pathway and represents a new genus within the siphoviridae family. / Morozova, Vera ; Jdeed, Ghadeer; Kozlova, Yuliya et al.

In: Viruses, Vol. 13, No. 7, 1372, 07.2021.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{316778ed3d294795bfa37bd7921d43b8,

title = "A new enterobacter cloacae bacteriophage ec151 encodes the deazaguanine dna modification pathway and represents a new genus within the siphoviridae family",

abstract = "A novel Enterobacter cloacae phage, EC151, was isolated and characterized. Electron microscopy revealed that EC151 has a siphovirus-like virion morphology. The EC151 nucleotide sequence shows limited similarity to other phage genomes deposited in the NCBI GenBank database. The size of the EC151 genome is 60,753 bp and contains 58 putative genes. Thirty-nine of them encode proteins of predicted function, 18 are defined as hypothetical proteins, and one ORF identifies as the tRNA-Ser-GCT-encoding gene. Six ORFs were predicted to be members of the deazaguanine DNA modification pathway, including the preQ0 transporter. Comparative proteomic phylogenetic analysis revealed that phage EC151 represents a distinct branch within a group of sequences containing clades formed by members of the Seuratvirus, Nonagvirus, and Vidquintavirus genera. In addition, the EC151 genome showed gene synteny typical of the Seuratvirus, Nonagvirus, and Nipunavirus phages. The average genetic distances of EC151/Seuratvirus, EC151/Nonagvirus, and EC151/Vidquintavirus are approximately equal to those between the Seuratvirus, Nonagvirus, and Vidquintavirus genera (~0.7 substitutions per site). Therefore, EC151 may represent a novel genus within the Siphoviridae family. The origin of the deazaguanine DNA modification pathway in the EC151 genome can be traced to Escherichia phages from the Seuratvirus genus.",

keywords = "Deazaguanine modification pathway, Enterobacter phage, Nonagvirus, PreQ0 transporter, Seuratvirus, Enterobacter cloacae/genetics, Viral Proteins/genetics, Genomics, Siphoviridae/genetics, DNA, Viral/genetics, Host Specificity, Virion/metabolism, Phylogeny, Bacteriophages/genetics, Proteomics, Genome, Viral/genetics",

author = "Vera Morozova and Ghadeer Jdeed and Yuliya Kozlova and Igor Babkin and Artem Tikunov and Nina Tikunova",

note = "Funding Information: Funding: The study was supported by the Russian Scientific Foundation, Project No. 21-14-00360. The Collection of Extremophile Microorganisms and Type Cultures of ICBFM SB RAS was supported by the Ministry of Education and Science of the Russian Federation, Project No. 121031300043-8. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",

year = "2021",

month = jul,

doi = "10.3390/v13071372",

language = "English",

volume = "13",

journal = "Viruses",

issn = "1999-4915",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "7",

}

RIS

TY - JOUR

T1 - A new enterobacter cloacae bacteriophage ec151 encodes the deazaguanine dna modification pathway and represents a new genus within the siphoviridae family

AU - Morozova, Vera

AU - Jdeed, Ghadeer

AU - Kozlova, Yuliya

AU - Babkin, Igor

AU - Tikunov, Artem

AU - Tikunova, Nina

N1 - Funding Information: Funding: The study was supported by the Russian Scientific Foundation, Project No. 21-14-00360. The Collection of Extremophile Microorganisms and Type Cultures of ICBFM SB RAS was supported by the Ministry of Education and Science of the Russian Federation, Project No. 121031300043-8. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.

PY - 2021/7

Y1 - 2021/7

N2 - A novel Enterobacter cloacae phage, EC151, was isolated and characterized. Electron microscopy revealed that EC151 has a siphovirus-like virion morphology. The EC151 nucleotide sequence shows limited similarity to other phage genomes deposited in the NCBI GenBank database. The size of the EC151 genome is 60,753 bp and contains 58 putative genes. Thirty-nine of them encode proteins of predicted function, 18 are defined as hypothetical proteins, and one ORF identifies as the tRNA-Ser-GCT-encoding gene. Six ORFs were predicted to be members of the deazaguanine DNA modification pathway, including the preQ0 transporter. Comparative proteomic phylogenetic analysis revealed that phage EC151 represents a distinct branch within a group of sequences containing clades formed by members of the Seuratvirus, Nonagvirus, and Vidquintavirus genera. In addition, the EC151 genome showed gene synteny typical of the Seuratvirus, Nonagvirus, and Nipunavirus phages. The average genetic distances of EC151/Seuratvirus, EC151/Nonagvirus, and EC151/Vidquintavirus are approximately equal to those between the Seuratvirus, Nonagvirus, and Vidquintavirus genera (~0.7 substitutions per site). Therefore, EC151 may represent a novel genus within the Siphoviridae family. The origin of the deazaguanine DNA modification pathway in the EC151 genome can be traced to Escherichia phages from the Seuratvirus genus.

AB - A novel Enterobacter cloacae phage, EC151, was isolated and characterized. Electron microscopy revealed that EC151 has a siphovirus-like virion morphology. The EC151 nucleotide sequence shows limited similarity to other phage genomes deposited in the NCBI GenBank database. The size of the EC151 genome is 60,753 bp and contains 58 putative genes. Thirty-nine of them encode proteins of predicted function, 18 are defined as hypothetical proteins, and one ORF identifies as the tRNA-Ser-GCT-encoding gene. Six ORFs were predicted to be members of the deazaguanine DNA modification pathway, including the preQ0 transporter. Comparative proteomic phylogenetic analysis revealed that phage EC151 represents a distinct branch within a group of sequences containing clades formed by members of the Seuratvirus, Nonagvirus, and Vidquintavirus genera. In addition, the EC151 genome showed gene synteny typical of the Seuratvirus, Nonagvirus, and Nipunavirus phages. The average genetic distances of EC151/Seuratvirus, EC151/Nonagvirus, and EC151/Vidquintavirus are approximately equal to those between the Seuratvirus, Nonagvirus, and Vidquintavirus genera (~0.7 substitutions per site). Therefore, EC151 may represent a novel genus within the Siphoviridae family. The origin of the deazaguanine DNA modification pathway in the EC151 genome can be traced to Escherichia phages from the Seuratvirus genus.

KW - Deazaguanine modification pathway

KW - Enterobacter phage

KW - Nonagvirus

KW - PreQ0 transporter

KW - Seuratvirus

KW - Enterobacter cloacae/genetics

KW - Viral Proteins/genetics

KW - Genomics

KW - Siphoviridae/genetics

KW - DNA, Viral/genetics

KW - Host Specificity

KW - Virion/metabolism

KW - Phylogeny

KW - Bacteriophages/genetics

KW - Proteomics

KW - Genome, Viral/genetics

UR - http://www.scopus.com/inward/record.url?scp=85111118958&partnerID=8YFLogxK

U2 - 10.3390/v13071372

DO - 10.3390/v13071372

M3 - Article

C2 - 34372577

AN - SCOPUS:85111118958

VL - 13

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 7

M1 - 1372

ER -

ID: 29238907