Research output: Contribution to journal › Article › peer-review
A Neurotoxic Insecticide Promotes Fungal Infection in Aedes aegypti Larvae by Altering the Bacterial Community. / Noskov, Y. A.; Kabilov, M. R.; Polenogova, O. V. et al.
In: Microbial Ecology, Vol. 81, No. 2, 02.2021, p. 493-505.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - A Neurotoxic Insecticide Promotes Fungal Infection in Aedes aegypti Larvae by Altering the Bacterial Community
AU - Noskov, Y. A.
AU - Kabilov, M. R.
AU - Polenogova, O. V.
AU - Yurchenko, Y. A.
AU - Belevich, O. E.
AU - Yaroslavtseva, O. N.
AU - Alikina, T. Y.
AU - Byvaltsev, A. M.
AU - Rotskaya, U. N.
AU - Morozova, V. V.
AU - Glupov, V. V.
AU - Kryukov, V. Y.
N1 - Publisher Copyright: © 2020, Springer Science+Business Media, LLC, part of Springer Nature. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2
Y1 - 2021/2
N2 - Symbiotic bacteria have a significant impact on the formation of defensive mechanisms against fungal pathogens and insecticides. The microbiome of the mosquito Aedes aegypti has been well studied; however, there are no data on the influence of insecticides and pathogenic fungi on its structure. The fungus Metarhizium robertsii and a neurotoxic insecticide (avermectin complex) interact synergistically, and the colonization of larvae with hyphal bodies is observed after fungal and combined (conidia + avermectins) treatments. The changes in the bacterial communities (16S rRNA) of Ae. aegypti larvae under the influence of fungal infection, avermectin toxicosis, and their combination were studied. In addition, we studied the interactions between the fungus and the predominant cultivable bacteria in vitro and in vivo after the coinfection of the larvae. Avermectins increased the total bacterial load and diversity. The fungus decreased the diversity and insignificantly increased the bacterial load. Importantly, avermectins reduced the relative abundance of Microbacterium (Actinobacteria), which exhibited a strong antagonistic effect towards the fungus in in vitro and in vivo assays. The avermectin treatment led to an increased abundance of Chryseobacterium (Flavobacteria), which exerted a neutral effect on mycosis development. In addition, avermectin treatment led to an elevation of some subdominant bacteria (Pseudomonas) that interacted synergistically with the fungus. We suggest that avermectins change the bacterial community to favor the development of fungal infection.
AB - Symbiotic bacteria have a significant impact on the formation of defensive mechanisms against fungal pathogens and insecticides. The microbiome of the mosquito Aedes aegypti has been well studied; however, there are no data on the influence of insecticides and pathogenic fungi on its structure. The fungus Metarhizium robertsii and a neurotoxic insecticide (avermectin complex) interact synergistically, and the colonization of larvae with hyphal bodies is observed after fungal and combined (conidia + avermectins) treatments. The changes in the bacterial communities (16S rRNA) of Ae. aegypti larvae under the influence of fungal infection, avermectin toxicosis, and their combination were studied. In addition, we studied the interactions between the fungus and the predominant cultivable bacteria in vitro and in vivo after the coinfection of the larvae. Avermectins increased the total bacterial load and diversity. The fungus decreased the diversity and insignificantly increased the bacterial load. Importantly, avermectins reduced the relative abundance of Microbacterium (Actinobacteria), which exhibited a strong antagonistic effect towards the fungus in in vitro and in vivo assays. The avermectin treatment led to an increased abundance of Chryseobacterium (Flavobacteria), which exerted a neutral effect on mycosis development. In addition, avermectin treatment led to an elevation of some subdominant bacteria (Pseudomonas) that interacted synergistically with the fungus. We suggest that avermectins change the bacterial community to favor the development of fungal infection.
KW - Avermectins
KW - Entomopathogens
KW - Metarhizium
KW - Microbiome
KW - Mosquito
KW - ENTOMOPATHOGENIC FUNGI
KW - AVERMECTINS
KW - MECHANISMS
KW - GUT MICROBIOTA
KW - LARVAL
KW - METARHIZIUM-ANISOPLIAE
KW - DYNAMICS
KW - DIVERSITY
KW - BIOLOGICAL-CONTROL
KW - AEROMONAS
UR - http://www.scopus.com/inward/record.url?scp=85089742966&partnerID=8YFLogxK
U2 - 10.1007/s00248-020-01567-w
DO - 10.1007/s00248-020-01567-w
M3 - Article
C2 - 32839879
AN - SCOPUS:85089742966
VL - 81
SP - 493
EP - 505
JO - Microbial Ecology
JF - Microbial Ecology
SN - 0095-3628
IS - 2
ER -
ID: 24999938