Research output: Contribution to journal › Article › peer-review
A highly versatile automatized setup for quantitative measurements of PHIP enhancements. / Kiryutin, Alexey S.; Sauer, Grit; Hadjiali, Sara et al.
In: Journal of Magnetic Resonance, Vol. 285, 01.12.2017, p. 26-36.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - A highly versatile automatized setup for quantitative measurements of PHIP enhancements
AU - Kiryutin, Alexey S.
AU - Sauer, Grit
AU - Hadjiali, Sara
AU - Yurkovskaya, Alexandra V.
AU - Breitzke, Hergen
AU - Buntkowsky, Gerd
N1 - Copyright © 2017 Elsevier Inc. All rights reserved.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - The design and application of a versatile and inexpensive experimental extension to NMR spectrometers is described that allows to carry out highly reproducible PHIP experiments directly in the NMR sample tube, i.e. under PASADENA condition, followed by the detection of the NMR spectra of hyperpolarized products with high spectral resolution. Employing this high resolution it is feasible to study kinetic processes in the solution with high accuracy. As a practical example the dissolution of hydrogen gas in the liquid and the PHIP kinetics during the hydrogenation reaction of Fmoc-O-propargyl-L-tyrosine in acetone-d6 are monitored. The timing of the setup is fully controlled by the pulse-programmer of the NMR spectrometer. By flushing with an inert gas it is possible to efficiently quench the hydrogenation reaction in a controlled fashion and to detect the relaxation of hyperpolarization without a background reaction. The proposed design makes it possible to carry out PHIP experiments in an automatic mode and reliably determine the enhancement of polarized signals.
AB - The design and application of a versatile and inexpensive experimental extension to NMR spectrometers is described that allows to carry out highly reproducible PHIP experiments directly in the NMR sample tube, i.e. under PASADENA condition, followed by the detection of the NMR spectra of hyperpolarized products with high spectral resolution. Employing this high resolution it is feasible to study kinetic processes in the solution with high accuracy. As a practical example the dissolution of hydrogen gas in the liquid and the PHIP kinetics during the hydrogenation reaction of Fmoc-O-propargyl-L-tyrosine in acetone-d6 are monitored. The timing of the setup is fully controlled by the pulse-programmer of the NMR spectrometer. By flushing with an inert gas it is possible to efficiently quench the hydrogenation reaction in a controlled fashion and to detect the relaxation of hyperpolarization without a background reaction. The proposed design makes it possible to carry out PHIP experiments in an automatic mode and reliably determine the enhancement of polarized signals.
KW - Gas-dissolution
KW - Hyperpolarization
KW - Kinetics
KW - Parahydrogen Induced Polarization
KW - SIGNAL ENHANCEMENT
KW - CONTRAST AGENTS
KW - NUCLEAR-MAGNETIC-RESONANCE
KW - SPIN HYPERPOLARIZATION
KW - REVERSIBLE EXCHANGE
KW - HYDROGEN
KW - SPECTROSCOPY
KW - SABRE
KW - PARAHYDROGEN-INDUCED POLARIZATION
KW - STATE NMR CHARACTERIZATION
UR - http://www.scopus.com/inward/record.url?scp=85032871189&partnerID=8YFLogxK
U2 - 10.1016/j.jmr.2017.10.007
DO - 10.1016/j.jmr.2017.10.007
M3 - Article
C2 - 29073504
AN - SCOPUS:85032871189
VL - 285
SP - 26
EP - 36
JO - Journal of Magnetic Resonance
JF - Journal of Magnetic Resonance
SN - 1090-7807
ER -
ID: 9410006