Research output: Contribution to journal › Article › peer-review
Встречаемость эндосимбиотической бактерии рода Wolbachia в природных популяциях Ostrinia Nubilalis и Ostrinia Scapulalis (Lepidoptera: Pyraloidea: Crambidae) на Юго-Западе России. / Tokarev, Y. S.; Yudina, M. A.; Malysh, J. M. et al.
In: Экологическая генетика, Vol. 15, No. 1, 6, 2017, p. 44-49.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Встречаемость эндосимбиотической бактерии рода Wolbachia в природных популяциях Ostrinia Nubilalis и Ostrinia Scapulalis (Lepidoptera: Pyraloidea: Crambidae) на Юго-Западе России
AU - Tokarev, Y. S.
AU - Yudina, M. A.
AU - Malysh, J. M.
AU - Bykov, R. A.
AU - Frolov, A. N.
AU - Grushevaya, I. V.
AU - Ilinsky, Y. Y.
N1 - Токарев Ю.С., Юдина М.А., Малыш Ю.М., и др. Встречаемость эндосимбиотической бактерии рода Wolbachia в природных популяциях Ostrinia Nubilalis и Ostrinia Scapulalis (Lepidoptera: Pyraloidea: Crambidae) на Юго-Западе России // Экологическая генетика. - 2017. - Т. 15. - № 1. - C. 44-49
PY - 2017
Y1 - 2017
N2 - Endosymbiotic bacteria of the genus Wolbachia are widespread in arthropods and often cause reproductive abnormalities in lepidopteran insects, including corn borers of the genus Ostrinia. Wolbachia-Ostrinia is apromising modelor studies of parasite-hostinter - actions yet parasite prevalence in natural insect host populations remains unk now n. Materials and Methods: Molecular genetic screening and statistical analysis is applied to evaluate prevalence rates of Wolbachia in sympatric populations of two corn borer species. Individual genomic DNA samples were extracted from last instar larvae collected in nature from different forage plants. For each sample of DNA showing positive signal with insect-specific primers the detection is performed using three diagnostic loci of Wolbachia: 16SrRNA, gatB and fbpA. Results: Wolbachia-positive signal is obtained for 13.5% larvae of Ostrinia nubilalis (N = 141) and 31.9% larvae of Ostrinia scapulalis (N = 138). In different localities the Wolbachia prevalence ranged from 2.9% (N = 34) to 65.8% (N = 38). Significantly higher rates of Wolbachia prevalence in insects from mugwort and hemp (O. scapulalis) as compared to those from corn (O. nubilalis) are revealed in three out of four localities. Conclusions: Endosymbiotic bacteria of the genus Wolbachia are revealed in natural populations of corn borers for the first time for Eastern Europe. The prevalence rates can be high and this should be taken into consideration when reproductive isolation is exa mined in population of these hosts as well as establishment of laboratory cultures is performed.
AB - Endosymbiotic bacteria of the genus Wolbachia are widespread in arthropods and often cause reproductive abnormalities in lepidopteran insects, including corn borers of the genus Ostrinia. Wolbachia-Ostrinia is apromising modelor studies of parasite-hostinter - actions yet parasite prevalence in natural insect host populations remains unk now n. Materials and Methods: Molecular genetic screening and statistical analysis is applied to evaluate prevalence rates of Wolbachia in sympatric populations of two corn borer species. Individual genomic DNA samples were extracted from last instar larvae collected in nature from different forage plants. For each sample of DNA showing positive signal with insect-specific primers the detection is performed using three diagnostic loci of Wolbachia: 16SrRNA, gatB and fbpA. Results: Wolbachia-positive signal is obtained for 13.5% larvae of Ostrinia nubilalis (N = 141) and 31.9% larvae of Ostrinia scapulalis (N = 138). In different localities the Wolbachia prevalence ranged from 2.9% (N = 34) to 65.8% (N = 38). Significantly higher rates of Wolbachia prevalence in insects from mugwort and hemp (O. scapulalis) as compared to those from corn (O. nubilalis) are revealed in three out of four localities. Conclusions: Endosymbiotic bacteria of the genus Wolbachia are revealed in natural populations of corn borers for the first time for Eastern Europe. The prevalence rates can be high and this should be taken into consideration when reproductive isolation is exa mined in population of these hosts as well as establishment of laboratory cultures is performed.
KW - Corn borers
KW - Forage plants
KW - Lepidoptera
KW - Molecular genetic detection
KW - Wolbachia
UR - http://www.scopus.com/inward/record.url?scp=85036648641&partnerID=8YFLogxK
UR - https://www.elibrary.ru/item.asp?id=29078393
U2 - 10.17816/ecogen15144-49
DO - 10.17816/ecogen15144-49
M3 - статья
AN - SCOPUS:85036648641
VL - 15
SP - 44
EP - 49
JO - Экологическая генетика
JF - Экологическая генетика
SN - 1811-0932
IS - 1
M1 - 6
ER -
ID: 9029807