TY - JOUR

T1 - Биологическое тестирование никелидтитановых имплантатов с покрытиями из благородных металлов в эксперименте in vivo

AU - Zheravin, Alexander

AU - Dorovskikh, Svetlana I.

AU - Vikulova, Eugenia S.

AU - Basova, Tamara V.

AU - Vasileva, Maria B.

AU - Rusakova, Yanina L.

AU - Morozova, Nataliya B.

N1 - This research was funded by the Russian Science Foundation for the funding project N 20-15-00222-P. Conflict of interests The authors declare that they have no conflict of interest. Compliance with Ethical Standards The study was conducted in accordance with ethical principles outlined in the Declaration of Helsinki approved by Ethics Committee of E.N. Meshalkin National Medical Research Center of the Ministry of Health of Russia (15, Rechkunovskaya St., Novosibirsk, 630055, Russia), protocol No. 1 dated April 22, 2022. The work was carried out in accordance with ethical standards and international and Russian regulatory documents (European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes: EST No. 123 dated 18.03.1986, Strasbourg; Order of the Ministry of Health of the Russian Federation No. 199n dated 01.04.2016; Interstate standard GOST 33044-2014; Euroguide on the accommodation and care of animals used for experimental and other scientific purposes. 2007. FELASA: Federation of European Laboratory Animal Science Associations). Acknowledgment The authors thank the Ministry of Education and Science of the Russian Federation for access to the equipment of the Collaborative Use Center and the Laboratory for Medical Alloys and Shape Memory Implants, Siberian Physical-Technical Institute (Tomsk) for providing the initial TiNi samples for research.

PY - 2024/1/1

Y1 - 2024/1/1

N2 - The aim of study is to conduct in vivo biological testing of titanium nickelide samples modified with Ag/Pt or AuAg/Pt film heterostructures in comparison with the bare carrier. Material and Methods. Titanium nickelide plates modified with film heterostructures made of noble metals and the laboratory mini-pigs used for in vivo tests were the objects of the study. To form film structures on titanium nickelide samples, the physical gas-phase deposition methods: ion plasma deposition (IPD) and thermal (PVD) sputtering were used. The Ag/ Pt or AuAg/Pt heterostructures were characterized by X-ray diffraction and scanning microscopy methods. Results. The biocompatibility of implants before (TiNi, control) and after (Ag/Pt/TiNi and AuAg/Pt/TiNi) modification with film heterostructures was tested in in-vivo experiments on a laboratory animal (mini-pig). General toxic reactions of the body to the injected samples were absent. A comparative macroscopic and histological analysis of the condition of peri-implant tissues after 39 days of implantation was performed. The connective tissue capsule around the TiNi sample revealed the presence of a certain number of lymphocytes, eosinophils and macrophages, but these indicators decrease in the order of TiNi > AuAg/Pt/TiNi > Ag/Pt/TiNi. Conclusion. The positive effect of modifying the titanium nickelide surfaces with noble metal heterostructures on the biocompatibility of metal implants was demonstrated in an in vivo experiment.

AB - The aim of study is to conduct in vivo biological testing of titanium nickelide samples modified with Ag/Pt or AuAg/Pt film heterostructures in comparison with the bare carrier. Material and Methods. Titanium nickelide plates modified with film heterostructures made of noble metals and the laboratory mini-pigs used for in vivo tests were the objects of the study. To form film structures on titanium nickelide samples, the physical gas-phase deposition methods: ion plasma deposition (IPD) and thermal (PVD) sputtering were used. The Ag/ Pt or AuAg/Pt heterostructures were characterized by X-ray diffraction and scanning microscopy methods. Results. The biocompatibility of implants before (TiNi, control) and after (Ag/Pt/TiNi and AuAg/Pt/TiNi) modification with film heterostructures was tested in in-vivo experiments on a laboratory animal (mini-pig). General toxic reactions of the body to the injected samples were absent. A comparative macroscopic and histological analysis of the condition of peri-implant tissues after 39 days of implantation was performed. The connective tissue capsule around the TiNi sample revealed the presence of a certain number of lymphocytes, eosinophils and macrophages, but these indicators decrease in the order of TiNi > AuAg/Pt/TiNi > Ag/Pt/TiNi. Conclusion. The positive effect of modifying the titanium nickelide surfaces with noble metal heterostructures on the biocompatibility of metal implants was demonstrated in an in vivo experiment.

KW - X-ray study

KW - antibacterial agent

KW - coating

KW - gold

KW - histological study

KW - implant

KW - implantation

KW - platinum

KW - silver

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85211102684&origin=inward&txGid=74c7293cc8b1fa5f7d17063f4d7d8e97

UR - https://www.mendeley.com/catalogue/c44ee993-d801-357a-9dde-1f4cd59a21b2/

U2 - 10.21294/1814-4861-2024-23-5-73-84

DO - 10.21294/1814-4861-2024-23-5-73-84

M3 - Article

VL - 23

SP - 73

EP - 84

JO - Siberian Journal of Oncology

JF - Siberian Journal of Oncology

SN - 1814-4861

IS - 5

ER -